Transgenosis oilseed rape SD-rapeseed capable of polymerizing common screening targets and application of transgenosis oilseed rape SD-rapeseed

Abstract

The invention discloses transgenosis oilseed rape SD-rapeseed capable of polymerizing common screening targets and application of the transgenosis oilseed rape SD-rapeseed, and relates to the field of biotechnology including genetic transformation and transgenic test. In the invention, eleven common transgenosis screening elements are guided into double No. 6 in conventional rapeseed varieties through an agrobacterium-mediated method, so that the transgenosis oilseed rape SD-rapeseed is obtained. The fusion sequence of the eleven inserted transgenosis screening elements is shown as SEQ NO.1. The transgenosis oilseed rape SD-rapeseed is taken as a raw material, so that a transgenosis oilseed rape screening detection general base standard substance and a genome DNA standard substance can be prepared. The transgenosis oilseed rape SD-rapeseed is applicable to transgenosis oilseed rape screening detection and preparation of screening detection standard substances, and overcomes the defect that the pollution is easy to pollute when plasmid standard substances are prepared.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a transgenic rapeseed SD-rapeseed that aggregates commonly used screening targets and its application, specifically, a transgenic rapeseed that aggregates 11 commonly used screening targets and its application in transgenic standards obtained through genetic transformation. Applications in substance preparation and transgenic detection. Background technique [0002] Since the large-scale commercialization of genetically modified crops, the safety of genetically modified organisms has been concerned by all walks of life. For this reason, more than 50 countries and regions, including China, the European Union, Japan and South Korea, have promulgated and implemented the safety management of genetically modified organisms. Laws and regulations; in order to protect consumers' right to know, the labeling system has been implemented for genetically modified products, and the labelin...

AI Technical Summary

Problems solved by technology

However, there are many problems in transgenic screening using a mixture of standard substances for specific detection of transformation events: 1) There are no corresponding standard substances for transgenic elements such as the hygromycin resistance gene HPT commonly used in genetic engineering research; 2) Some screening elements It occurs repeatedly in different transformation events, resulting in inconsistent copy numbers of different elements in the positive control, which makes it difficult to accurately reflect the sensitivity of detection; 3) There are differences in the DNA extraction efficiency of different transgenic components, resulting in errors in the judgment of detection sensitivity

[0006] After searching the existing patents and other documents, no reports on the development and application of general reference materials for the screening and detection of transgenic rapeseed have been found.

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