A kind of preparation method of alitame standard substance

A standard substance, Alitame technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome procedures, high cost, long delivery cycle, etc., and achieve simple process, stable product quality, and good uniformity Effect

CN105717240BInactive Publication Date: 2018-01-30CHINA NAT INST OF STANDARDIZATION
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2018-01-30
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for preparing Alitame standard substance. The method comprises the first step of test solution preparing, the second step of liquid chromatogram preparing and the third step of collecting. A semi-preparative liquid chromatogram system is adopted, the Alitame standard sample prepared in a separated mode is high in purity, the technology is simple, the preparing speed is high, the product quality is stable, uniformity is good, high purity (99% or above) can be acquired, the related solvent is free of pollution, and clean production can be achieved.
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Description

technical field

[0001] The invention relates to a method for preparing alitame standard substance, belonging to the field of compound extraction and purification. Background technique

[0002] Sweetener is an important raw material in the food industry and an auxiliary material in the pharmaceutical industry. Sucrose and glucose are commonly used natural sweeteners. However, due to the high calories contained in sugar, it is easy to make the eater fat, and it is easy to induce many diseases, such as cardiovascular disease and diabetes; eat. For this reason, various synthetic sweeteners have been developed, which are mainly aimed at the characteristics of low calorie, high sweetness, safety to the human body, good stability, and convenience for food processing, and have become indispensable food additives in people's lives. Alitame is a dipeptide sweetener, its sweetness is more than 2000 times that of sucrose, it is very stable in acid, heat and other conditions, it has r...

Examples

Embodiment 1

[0025] A method for preparing an alitame standard substance, comprising the following steps:

[0026] (1) Preparation of alitame solution: the raw material for preparing alitame standard substance is industrial grade alitame, which is dissolved in water and dissolved by ultrasonic waves, and then filtered through a 0.22 μm filter membrane to obtain alitame solution;

[0027] (2) Liquid chromatography preparation: inject alitame solution into preparative chromatography to separate pure fractions, and the chromatographic conditions are that the chromatographic column is PREP-ODS(H) KIT (250mm×20mm, 5 μm); mobile phase: 40% (v / v) methanol aqueous solution; flow rate: 10mL / min; column temperature: 30°C; running time: 15min; detection wavelength: 210nm, injection volume 0.2mL, collect fractions within 7.5-9min;

[0028] (3) Fraction collection: the fractions were received in a brown bottle wrapped with tinfoil on the outer wall filled with nitrogen in advance, and then neutralize...

Embodiment 2

[0033] A method for preparing an alitame standard substance, comprising the following steps:

[0034] (1) Preparation of the test solution: the raw material for the preparation of the alitame standard substance is technical grade alitame, the alitame raw material is dissolved in water and dissolved by ultrasound, and then filtered through a 0.22 μm filter membrane to obtain the test substance solution;

[0035] (2) Liquid chromatographic preparation: inject the initial extract of alitame into the preparative chromatographic separation of pure fractions, and the chromatographic conditions are that the chromatographic column is WatersXTerraRP18 (250mm×20mm, 5μm); mobile phase: 40% (v / v) aqueous methanol ;Flow rate: 10mL / min; Column temperature: 30°C; Running time: 15min; Detection wavelength: 210nm, injection volume 0.5mL to collect fractions within 4.5-5.5min;

[0036] (3) Collection: The distillate was received in a brown bottle wrapped with tinfoil on the outer wall filled w...

Embodiment 3

[0040] A method for preparing an alitame standard substance, comprising the following steps:

[0041] (1) Preparation of the test solution: the raw material for the preparation of the alitame standard substance is technical grade alitame, the alitame raw material is dissolved in water and dissolved by ultrasound, and then filtered through a 0.22 μm filter membrane to obtain the test substance solution;

[0042] (2) Liquid chromatographic preparation: the initial extraction of alitame is injected into the preparative chromatographic separation of pure fractions, and the chromatographic conditions are that the chromatographic column is PREP-ODS(H) KIT (250mm×20mm, 5μm); mobile phase: 60% (v / v) methanol aqueous solution; flow rate: 10mL / min; column temperature: 30°C; running time: 15min; detection wavelength: 210nm, injection volume 0.5mL to collect fractions within 7.5-9min;

[0043] (3) Collection: The distillate was received in a brown bottle wrapped with tinfoil on the outer w...