Multicolor fluorescence imaging method and system under single exposure

A fluorescence imaging, single-exposure technology, used in fluorescence/phosphorescence, material analysis by optical means, measurement devices, etc., can solve the problems of reduced spatial resolution, high spectral resolution, difficult to obtain, etc. Effect

Active Publication Date: 2017-06-13
TSINGHUA UNIV
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Problems solved by technology

[0004] The main disadvantage of current multispectral microscopy techniques is the sacrifice of spatial and temporal resolution
In this regard, different narrow-band filters can be added in front of the sensor each time for collection. This method will increase the collection time and it is difficult to obtain high spectral resolution.
It is possible to group the camera pixels and use a grating to split the light, but this technique reduces the spatial resolution by the same factor as the spectral resolution

Method used

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  • Multicolor fluorescence imaging method and system under single exposure
  • Multicolor fluorescence imaging method and system under single exposure

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Embodiment Construction

[0020] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary only for explaining the present invention and should not be construed as limiting the present invention.

[0021] In describing the present invention, it should be understood that the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", " The orientations or positional relationships indicated by "vertical", "horizontal", "top", "bottom", "inner" and "outer" are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and Simplified descriptions, rather than indicating or implying that the device or element refe...

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Abstract

The invention discloses a multicolor fluorescence imaging method and system under single exposure. The method comprises the steps of amplifying a fluorescence sample on an image plane through an object lens to obtain a first real image on a focal plane of the object lens; arranging a reflector on the focal plane of the object lens and carrying out spectrum separation on the first real image through a 4f system and a grating arranged in the 4f system; obtaining an image after spectrum separation through a collection device, wherein the reflector is arranged on the front focal plane of the 4f system and the grafting is arranged on the focal plane in the middle of the 4f system. The multicolor fluorescence imaging method and system have the advantage that the imaging rate of a multispectral fluorescence microscopy is improved.

Description

technical field [0001] The invention relates to the field of computational imaging, in particular to a method and system for multicolor fluorescence imaging under single exposure. Background technique [0002] Fluorescence microscopy has a wide range of applications in the life sciences. For example, when studying the transfer characteristics of a certain living structure, it is usually necessary to implant a certain fluorescent protein into it and track it. At the same time, changes in the autofluorescence of life structures can also reflect changes in the nature of life to some extent. Therefore, research on fluorescence microscopy will be able to greatly promote the development of life sciences. [0003] The current fluorescence microscopy techniques mainly include confocal fluorescence microscopy, two-photon fluorescence microscopy, etc., which use a specific laser to excite the fluorescence of the sample set, and use a camera sensor at the acquisition end to collect f...

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6458G01N21/6486G01N2021/6463
Inventor 戴琼海陈星晔吴嘉敏
Owner TSINGHUA UNIV
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