The invention relates to an image scanning
microscopic imaging method and device based on a double-
microlens array, and belongs to the technical field of optical microscopic measurement. According tothe invention, an array type multi-focus generated by the micro-
lens array is used for illuminating a sample; the imaging speed is improved, the influence of out-of-focus
fluorescence is eliminated through the pinhole array, each focusing
light beam is scaled by 1 / 2 through the second
micro lens array, all-optical implementation of subsequent digital
information processing of traditional image
scanning microscopy is achieved, it is guaranteed that the resolution ratio is improved by two times, and meanwhile the problem that a traditional image
scanning microscopy system is low in imaging speedis solved. The
laser beam reaches the first micro-
lens array after being expanded to generate an array type illumination beam, and after the array type illumination beam is emitted to the surface ofa sample, the objective lens collects multi-focus
fluorescence from the sample and emits the multi-focus
fluorescence to the pinhole array to eliminate the influence of out-of-focus fluorescence. Thesecond micro-
lens array locally shrinks each focus point, meanwhile, the original focusing direction of the
light beam is kept, and the zoomed fluorescence focus is imaged to the sCMOS camera under the deflection effect of the scanning
galvanometer.