The invention discloses a long-term imaging device suitable for animal internal organs, including a suction cup, an
airway and an airtight cap. The suction cup is provided with a through adsorption hole, the upper end opening of the adsorption hole is sealed with a
glass slide, and the lower end is embedded in the
chest cavity or
abdominal cavity of the
experimental animal; the
airway is provided with a first end and a second end, the first end is connected to the adsorption hole, and the second end is detachably connected to a negative pressure source, the negative pressure source works to form a negative pressure area in the adsorption hole, and the animal internal organs are passively sucked into the adsorption hole and closely attached to the
glass slide; the airtight cap is detachably connected to the second end. The invention uses an airtight cap to isolate the outside world and the internal organs during observation when it is not suitable or during the observation interval, and uses negative pressure to partially suck the internal organs into the adsorption hole during observation. The negative pressure can avoid changes in the focal plane under the
microscope and
motion artifacts, and improve the stability of observation and detection; the observed part of the internal organs is adjusted by negative pressure, and the
field of view is changed within a certain range; and the
survival rate of animals in long-term continuous experiments is improved.