Targeted rat phospholipase Cgamma2 interfering recombinant adenovirus, and construction method and application thereof

A technology of recombinant adenovirus and rat phospholipids, which is applied in the field of biotechnology construction, can solve problems such as low efficiency, difficult gene therapy drugs, and difficult gene interference, so as to promote cell proliferation, prevent and treat liver failure, and achieve remarkable results Effect

Inactive Publication Date: 2017-06-20
HENAN UNIV OF SCI & TECH
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Although siRNA can efficiently and specifically inhibit gene expression in mammalian cells, the transfection efficiency of artificially synthesized RNA with...

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  • Targeted rat phospholipase Cgamma2 interfering recombinant adenovirus, and construction method and application thereof
  • Targeted rat phospholipase Cgamma2 interfering recombinant adenovirus, and construction method and application thereof
  • Targeted rat phospholipase Cgamma2 interfering recombinant adenovirus, and construction method and application thereof

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Embodiment Construction

[0042] The present invention will be described in detail below with reference to the drawings and specific embodiments, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. The test methods for which specific conditions are not indicated in the following examples are usually operated under conventional conditions, and the steps are not described in detail because they do not involve the invention point.

[0043] When the examples give numerical ranges, it should be understood that unless otherwise specified in the present invention, the two endpoints of each numerical range and any value between the two endpoints can be selected. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. In addition to the specific methods, equipment, and materials used in the embodiments, according to those skilled in the art's g...

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Abstract

The invention discloses a targeted rat phospholipase Cgamma2 interfering recombinant adenovirus and a construction method thereof. The targeted rat phospholipase Cgamma2 interfering recombinant adenovirus is obtained by inserting oligonucleotide shRNA (short hairpin ribonucleic acid) of targeted rat phospholipase Cgamma2 into an adenovirus genome-containing framework plasmid pHBAd-BHG, wherein the nucleotide sequence of the oligonucleotide shRNA is as shown in SEQ ID NO.1. Aiming at a siRNA (small interfering ribonucleic acid) nucleotide sequence of the rat phospholipase Cgamma2 gene, the invention designs and synthesizes the corresponding shRNA oligonucleotide sequence based on an adenovirus shuttle vector; the shRNA of the targeted target gene is continuously produced in a target cell by means of a U6 promoter of RNA (ribonucleic acid)-dependent polymerase III carried by the adenovirus vector itself; and the shRNA can be processed into the siRNA by Dicerase in the cell, thereby playing a gene interfering role.

Description

technical field [0001] The invention belongs to the field of biotechnology construction, and in particular relates to a recombinant adenovirus targeting rat phospholipase Cγ2 interference, a construction method and application. Background technique [0002] Acute liver failure is a group of clinical syndromes caused by severe liver damage caused by various factors, which in turn lead to serious dysfunction of the synthesis, detoxification, excretion and biotransformation of the liver itself. High (up to 80%), currently there is no specific treatment. The main pathological change is the massive death of liver cells. It has been found that one of the main forms of liver cell death in acute liver failure is apoptosis, and the occurrence of acute liver failure is closely related to liver cell apoptosis. Apoptosis, also known as programmed cell death, is an extremely complex process regulated by genes and plays an important role in the occurrence and development of liver failur...

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Application Information

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IPC IPC(8): C12N15/861A61K48/00A61P1/16
Inventor 陈晓光吕琼霞刘玉梅位治国张自强邓雯
Owner HENAN UNIV OF SCI & TECH
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