Gold label kit using NCAM-1 (neural cell adhesion molecule-1) as detection index and preparation method and application thereof

Abstract

The invention relates to a gold label kit using NCAM-1 (neural cell adhesion molecule-1) as a detection index, which aims at obtaining a kit for detecting activeness of lupus nephritis. The gold label kit comprises a substrate, a water absorbing pad, a nitrocellulose membrane, a gold label pad and a sample pad, wherein the sample pad, the gold label pad, the nitrocellulose membrane and the water absorbing pad are sequentially connected to and cover the substrate; one part of the gold label pad is covered by the sample pad; a detection line coated by any of mouse-anti-human / rabbit-anti-human NCAM-1 monoclonal antibody or rabbit-anti-human / sheep-anti-human NCAM-1 polyclonal antibody, and a detection line coated by any of sheep-anti-mouse immune globulin G and sheep-anti-rabbit immune globulin G polyclonal antibodies are arranged on the nitrocellulose membrane; the gold label pad is coated by a complex which is formed by coupling of any of colloidal gold and mouse-anti-human / rabbit-anti-human NCAM-1 monoclonal antibody. The kit is used for detecting the NCAM-1 concentration in a urea sample, so as to judge the activeness of lupus nephritis, thereby realizing quick and non-wound detection.

Description

technical field

[0001] The invention belongs to medical detection tools, and relates to a detection tool for lupus nephritis activity, in particular to a gold label kit using NCAM-1 as a detection index, its preparation method and application. Background technique

[0002] Lupus nephritis is one of the most common complications of systemic lupus erythematosus, and end-stage renal disease caused by lupus nephritis is the main factor leading to the death of patients with systemic lupus erythematosus. The main reason for developing from lupus nephritis to end-stage renal disease is that the active lupus nephritis is not detected in time, leading to untimely treatment, irreversible glomerulosclerosis and interstitial vascular fibrosis in the kidney. For the judgment of the activity of lupus nephritis, the "gold standard" is to conduct pathological examination of kidney tissue through renal biopsy, and make a diagnosis based on the pathological examination results. However, rena...

Examples

Embodiment 1

[0047] Application of a gold-labeled kit using NCAM-1 as a detection index in detecting the activity of lupus nephritis, wherein the gold-labeled kit detects the concentration of NCAM-1 in a urine sample as an index.

[0048] The above-mentioned gold standard kit for detecting lupus nephritis activity using NCAM-1 as a detection index includes a base plate 7, an absorbent pad 6, a nitrocellulose membrane 3, a gold standard pad and a sample pad 1, the sample pad 1, gold The standard pad 2, the nitrocellulose membrane 3 and the absorbent pad 6 are successively covered on the base plate 7, and the gold standard 2 pad is partially covered by the sample 1 pad; 1 detection line 4 coated with polyclonal antibody; control line 5 coated with goat anti-mouse immunoglobulin G polyclonal antibody, the gold standard pad 2 coated with colloidal gold and mouse anti-human NCAM-1 Conjugates formed by coupling monoclonal antibodies.

[0049] In this embodiment, the widths of the detection line...

Embodiment 2

[0064] The gold standard kit using NCAM-1 as a detection index, including a bottom plate 7, an absorbent pad 6, a nitrocellulose membrane 3, a gold standard pad 2 and a sample pad 1, the sample pad 1, gold standard pad 2, nitrocellulose The membrane 3 and the water-absorbing pad 6 are successively covered on the bottom plate 7, and the gold standard pad 2 is partially covered by the sample pad 1; The detection line 4 formed; the control line 5 formed by coating with goat anti-mouse immunoglobulin G), and the gold label pad 2 is coated with a conjugate formed by coupling colloidal gold and mouse anti-human NCAM-1 monoclonal antibody .

[0065] In this embodiment, the widths of the detection line 4 and the control line 5 are each controlled to be 0.5 mm, and the distance between the detection line 4 and the control line 5 is controlled to be 10 mm.

[0066] In this embodiment, the shape of the gold-labeled kit for detecting the activity of lupus nephritis is a rectangular bar. ...

Embodiment 3

[0084]The gold standard kit using NCAM-1 as a detection index includes a bottom plate 7, an absorbent pad 6, a nitrocellulose membrane, a gold standard pad and a sample pad, and the sample pad 1, the gold standard pad 2, the nitrocellulose membrane 3 and The water-absorbing pads 6 are successively covered on the base plate 7, and the gold standard pad 2 is partially covered by the sample pad 1; the nitrocellulose membrane 3 is provided with a detection line coated with a rabbit anti-human NCAM-1 polyclonal antibody 4. The control line 5 coated with goat anti-mouse immunoglobulin G, and the gold label pad 2 is coated with a conjugate formed by coupling colloidal gold and mouse anti-human NCAM-1 monoclonal antibody.

[0085] In this embodiment, the widths of the detection line 4 and the control line 5 are each controlled to be 0.5 mm, and the distance between the detection line 4 and the control line 5 is controlled to be 10 mm.

[0086] In this embodiment, the shape of the gold...

technical field

[0001] The invention belongs to medical detection tools, and relates to a detection tool for lupus nephritis activity, in particular to a gold label kit using NCAM-1 as a detection index, its preparation method and application. Background technique

[0002] Lupus nephritis is one of the most common complications of systemic lupus erythematosus, and end-stage renal disease caused by lupus nephritis is the main factor leading to the death of patients with systemic lupus erythematosus. The main reason for developing from lupus nephritis to end-stage renal disease is that the active lupus nephritis is not detected in time, leading to untimely treatment, irreversible glomerulosclerosis and interstitial vascular fibrosis in the kidney. For the judgment of the activity of lupus nephritis, the "gold standard" is to conduct pathological examination of kidney tissue through renal biopsy, and make a diagnosis based on the pathological examination results. However, rena...

Examples

Embodiment 1

[0047] Application of a gold-labeled kit using NCAM-1 as a detection index in detecting the activity of lupus nephritis, wherein the gold-labeled kit detects the concentration of NCAM-1 in a urine sample as an index.

[0048] The above-mentioned gold standard kit for detecting lupus nephritis activity using NCAM-1 as a detection index includes a base plate 7, an absorbent pad 6, a nitrocellulose membrane 3, a gold standard pad and a sample pad 1, the sample pad 1, gold The standard pad 2, the nitrocellulose membrane 3 and the absorbent pad 6 are successively covered on the base plate 7, and the gold standard 2 pad is partially covered by the sample 1 pad; 1 detection line 4 coated with polyclonal antibody; control line 5 coated with goat anti-mouse immunoglobulin G polyclonal antibody, the gold standard pad 2 coated with colloidal gold and mouse anti-human NCAM-1 Conjugates formed by coupling monoclonal antibodies.

[0049] In this embodiment, the widths of the detection line...

Embodiment 2

[0064] The gold standard kit using NCAM-1 as a detection index, including a bottom plate 7, an absorbent pad 6, a nitrocellulose membrane 3, a gold standard pad 2 and a sample pad 1, the sample pad 1, gold standard pad 2, nitrocellulose The membrane 3 and the water-absorbing pad 6 are successively covered on the bottom plate 7, and the gold standard pad 2 is partially covered by the sample pad 1; The detection line 4 formed; the control line 5 formed by coating with goat anti-mouse immunoglobulin G), and the gold label pad 2 is coated with a conjugate formed by coupling colloidal gold and mouse anti-human NCAM-1 monoclonal antibody .

[0065] In this embodiment, the widths of the detection line 4 and the control line 5 are each controlled to be 0.5 mm, and the distance between the detection line 4 and the control line 5 is controlled to be 10 mm.

[0066] In this embodiment, the shape of the gold-labeled kit for detecting the activity of lupus nephritis is a rectangular bar. ...

Embodiment 3

[0084]The gold standard kit using NCAM-1 as a detection index includes a bottom plate 7, an absorbent pad 6, a nitrocellulose membrane, a gold standard pad and a sample pad, and the sample pad 1, the gold standard pad 2, the nitrocellulose membrane 3 and The water-absorbing pads 6 are successively covered on the base plate 7, and the gold standard pad 2 is partially covered by the sample pad 1; the nitrocellulose membrane 3 is provided with a detection line coated with a rabbit anti-human NCAM-1 polyclonal antibody 4. The control line 5 coated with goat anti-mouse immunoglobulin G, and the gold label pad 2 is coated with a conjugate formed by coupling colloidal gold and mouse anti-human NCAM-1 monoclonal antibody.

[0085] In this embodiment, the widths of the detection line 4 and the control line 5 are each controlled to be 0.5 mm, and the distance between the detection line 4 and the control line 5 is controlled to be 10 mm.

[0086] In this embodiment, the shape of the gold...

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