Immunocolloidal gold rapid test product evaluation method for drug residue in aquatic product

Abstract

The invention discloses an immunocolloidal gold rapid test product evaluation method for a drug residue in an aquatic product, and relates to an immunocolloidal gold test strip/card quality evaluation method used for the residue of forbidden medicines, such as chloramphenicol, malachite green and nitrofuran metabolites. Based on confirmation of an immunocolloidal gold test strip/card qualitative method by taking forbidden medicines, such as chloramphenicol, malachite green and nitrofuran metabolites, as tested objects, a technician in a test strip/card production enterprise verifies a field test in a laboratory and an expert group reads the verification results and judges the quality and performance of the test strip/card. Parameters including sensitivity and specificity, actual detection rate of a positive sample, stability, matrix applicability and test time are verified in the field test. The method performs necessary technical evaluation on the product in an objective accurate way, so that it is ensured that a purchaser can buy the product meeting the performance index requirement.

Description

[0001] Technical field: [0002] The invention relates to an evaluation technology for immunocolloidal gold detection test strips / cards, in particular to a product evaluation method for rapid detection of immune colloidal gold residues in aquatic products. [0003] Background technique: [0004] The test strip / caine is easy to operate and the results can be obtained quickly. It exists as a rapid screening method in the analysis and testing work. Because most people agree with some methodological instability of the test strip / card method, such as False positive rate, false negative rate, poor stability between batches, etc. Almost all test strip / card methods need to use traditional detection methods as the basis for final confirmation when problems are found. Therefore, people ignore the more scientific and in-depth methodological performance index discussion on the test strip / card method, and this neglect is very dangerous in the analysis of some sensitive fields such as food s...

AI Technical Summary

Problems solved by technology

Foreign countries have carried out evaluation work on kits and made some progress. However, due to various reasons, almost all evaluation systems have some defects, mainly due to the wide variety of test strips / cards, which are sold in the market. There are various products of "test strips / cards", and the detection methods of different test strips / cards are quite different, which makes it quite technically difficult to construct the technical regulations of the evaluation system

So far, the management of test strips / cards has not been carried out in China, while foreign officials only use direct registration for management. One of the most successful evaluation agencies in the world, AFNOR, only evaluates kits for microbial detection, based on ISO16140 "Guidelines for the Verification of Microbiological Alternative Testing Methods for Food and Animal Feed", and another widely influential AOAC relies more on the strength of technical experts, with an expert group to determine the technical indicators that need to be verified

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