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An acid-alkali-resistant, alcohol-resistant strain of Lanxiella sugarcane and its application

A technology of Lancia cane, acid and alkali resistance, applied in the field of distiller's grain decomposing bacteria, can solve the problems of waste of manpower, material resources, financial resources and time, secondary pollution of distiller's grains to corrupt soil, low acid and alkali resistance of actinomycetes, etc., to achieve protection The environment and soil, the turbidity of the test tube is obvious, and the effect of preventing secondary pollution and corruption

Active Publication Date: 2019-10-25
陕西枫丹百丽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] The actinomycetes disclosed in the above-mentioned patent documents have low acid and alkali resistance, poor alcohol resistance, and unsatisfactory temperature resistance, and are still not suitable for direct high-temperature composting of liquor distiller's grains
[0014] In summary, although there are many high-temperature decomposing bacteria on liquor distiller's grains disclosed, regardless of single-bacteria fermentation or mixed-bacteria fermentation, it is necessary to reduce the alcohol content and acidity of the distiller's grains. The process is complicated and wastes manpower, material resources, financial resources and time. , also can cause secondary pollution corruption and soil alkalization of distiller's grains at the same time, pollute environment, and, along with the carrying out of high-temperature decomposing process, the pH value of distiller's grains increases, continues to ferment, and alcohol content can continue to increase (3-6% )

Method used

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  • An acid-alkali-resistant, alcohol-resistant strain of Lanxiella sugarcane and its application
  • An acid-alkali-resistant, alcohol-resistant strain of Lanxiella sugarcane and its application
  • An acid-alkali-resistant, alcohol-resistant strain of Lanxiella sugarcane and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation, purification, screening and identification of Laceyella sacchari (Laceyella sacchari) CP

[0037] 1) Separation and primary screening: Collect samples from high-temperature decomposed cow dung in Tangshan City, Hebei Province and bring them back to the laboratory. Take 10g of samples and add them to a sterilized Erlenmeyer flask with glass beads containing 100mL of cooled sterile water. Shake at 200r / min. The bed was oscillated and mixed for 30 minutes, then placed in a water bath at 80°C, and bathed in water for 10 minutes. -3 ~10 -9 Concentration, select 10 -3 , 10 -4 , 10 -5 , 10 -6 Use a pipette to draw 100 μL of each gradient suspension, spread it evenly on a nutrient agar plate (NA plate) with a spreader, use sterile water as a control, place the plate upside down in an incubator, and incubate at 50°C for 5 days , each treatment setting was repeated 3 groups horizontally, and according to the characteristics of microbial colony shape, siz...

Embodiment 2

[0068] Example 2 Test of tolerance to pH value of Laceyella sacchari (Laceyella sacchari) CP

[0069] Pick an appropriate amount of strains of Lancia saccharum CP slant and insert them into 100mL NA liquid medium, and cultivate them on a shaker at 50°C and 200r / min for 24 hours to obtain seed liquid. 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, 7.5, 8, 8.5, 9, 9.5, 10, 10.5, 11, 11.5, 12 in NA liquid medium , 50°C, 200r / min shaker culture for 3 days, spread the NA plate, observe the growth; then put the seed solution into the NA liquid medium test tube with the same pH gradient at 50°C, 200r / min Incubate on a shaker for 3 days and observe the turbidity.

[0070] Test results: Ransiella sugarcane CP was cultured in NA liquid medium with a pH value of 3-12 at 50°C for 3 days, and the coated NA plate could grow well; it was cultured in NA liquid medium test tube at 50°C and 200r / min shaking table After 3 days, the growth was good, and the turbidity was obvious. Both tests pr...

Embodiment 3

[0071] Example 3 Alcohol tolerance test of Laceyella sacchari (Laceyella sacchari) CP:

[0072] Pick an appropriate amount of strains of Lancia saccharum CP slant and inoculate them into 100mL NA liquid medium, and culture them on a shaker at 50°C and 200r / min for 24 hours to obtain seed liquid. In 1%, 3%, 5%, 7%, 9%, 11%, 13%, 15%, 20% NA liquid medium, culture at 50°C, 200r / min for 3 days, spread NA plates, and observe the growth ; Then, the seed solution was added to the NA liquid medium test tube with the same alcohol concentration gradient at 1‰ inoculation amount, cultured on a shaker at 50°C and 200r / min for 3 days, and the turbidity was observed.

[0073] Test results: Lanxiella sugarcane CP was cultured in NA liquid medium with an alcohol content of 1-13% at 50°C for 3 days, and the coated NA plate could grow well; in the NA liquid medium test tube at 50°C, shaken at 200r / min After 3 days of bed culture, it grows well, and the turbidity is obvious. Both tests have pr...

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Abstract

The invention discloses a strain of Laceyella sacchari CP which is acid-alkali-resistant and alcohol-resistant and rapidly decomposes distiller's grains, and its preservation number is: CGMCC NO.13928. The Lanciella sugarcane can grow in the range of 30-75°C, the optimum growth temperature is 60°C, and can produce various degradative enzymes such as protease and lipase; the alcohol content (V / V) is 1-13% It grows well with solid and liquid media with a pH value of 3-12, which is very suitable for the current dynamic high-temperature decomposition process characteristics of "pH value from low to high, alcohol content from high to low and then high" of liquor distiller's grains, without the need for distiller's grains The method of lowering the alcohol content and reducing the acidity reduces the processing procedures, saves manpower, material resources, financial resources and time, reaches 55°C in 1 day, reaches 85°C in 3 days, maintains 83-85°C for 3 days, and the fermentation cycle is only 15-16 days. It effectively improves the yield and quality of organic fertilizers, prevents secondary pollution and soil alkalinization of distiller's grains, protects the environment and soil, and opens up a new shortcut for the production of high-quality organic fertilizers by direct high-temperature composting of liquor distiller's grains.

Description

technical field [0001] The invention relates to distiller's lees decomposing bacteria, in particular to an acid-alkali-resistant and alcohol-resistant strain of Lancia saccharum rapidly decomposing distiller's grains and its application. Background technique [0002] Distiller's grains are the direct leftovers in the brewing process. At present, more than 50 million tons of distiller's grains need to be processed in my country. Therefore, people have been paying attention to the problem of disposing of distiller's grains after fermentation. Liquor distiller's grains are easily fermented and corrupted, and emit stench and easily pollute the environment. Because it contains a lot of crude fat, crude starch, crude protein and rich in nitrogen, phosphorus, potassium and polysaccharides, it is an excellent source of organic fertilizer. Therefore, the use of distiller's grains to make organic fertilizers can not only solve environmental problems, turn waste into treasure, but als...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/00C12R1/01
CPCC05F5/008C05F17/00C12N1/205C12R2001/01C05F11/08Y02W30/40
Inventor 刘辉王灵敏李玲娣
Owner 陕西枫丹百丽生物科技有限公司
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