Aerobic culture and gas collection integrated indoor soil culture apparatus, and application thereof

Abstract

The invention relates to an aerobic culture and gas collection integrated indoor soil culture apparatus, and an application thereof. The apparatus comprises a culture container, the top mouth of the culture container is provided with a separator plate matched with the top mouth, the separator plate comprises a separator plate body and a gas opening and closing device, the separator plate is in closed fixed connection with an aeration container with an open top end, the sidewall of the aeration container is provided with an aeration opening, the ventilation container is covered with a top cover, the top cover is provided with a gas inlet hole and a gas collecting hole, a gas inlet tube is fixed in the gas inlet hole, one end of the gas inlet tube is inserted into the culture container, the other end of the gas inlet tube is connected with a balancing tube, a gas collecting tube is fixed in the gas collecting hole, one end of the gas collecting tube is inserted into the culture container, and the other end of the gas collecting tube is connected with a gas collecting needle. The apparatus has the advantages of implementation of aerobic culture and gas collection on the apparatus, small size, use in a limited space, convenience in operation, realization of closed culture under normal pressure, quantification of the initial culture concentration of a target gas, solving of the problem of target gas absorption due to the negative pressure caused by sampling, and real and effective reality-approaching culture and detection result.

Description

technical field [0001] The invention relates to an indoor soil cultivation device integrating aeration cultivation and gas collection and its application, and belongs to the research technical field of soil simulation cultivation and greenhouse gas emission. Background technique [0002] Global warming has become the focus of worldwide attention, and its main driving factor is the enhancement of the greenhouse effect caused by the increase of greenhouse gas emissions in the atmosphere. carbon dioxide (CO 2 ), methane (CH 4 ) and nitrous oxide (N 2 O) are the three most important greenhouse gases in the atmosphere, and their contribution to global warming accounts for nearly 80%. It is estimated that 5%-20% of CO is in the atmosphere every year 2 , 15%-30% CH 4 and 80%-90% of N 2 O originates from the soil. Therefore, scholars at home and abroad have carried out relevant observations and research on the three types of greenhouse gas emissions in different ecosystem soi...

AI Technical Summary

Problems solved by technology

However, the device cannot quantify the initial culture concentration in the culture vessel, and the injection of air during the culture period will also affect the gas production and discharge in the culture vessel; in addition, the use of fine needles inserted into the silica gel plug to obtain air may easily cause needle blockage and long-term use of silica gel. Air plugging affects the accuracy of the test, which is also a common problem in the prior art

[0006] After searching, there is no publication of relevant literature on an indoor soil cultivation device that integrates aeration cultivation and gas collection that is more realistic, effective and closer to reality.

Images