Strain of auricularia auricula

A technology of black fungus and strains, applied in the direction of fungi, microorganisms, biochemical equipment and methods, etc., can solve the problems of poor quality, small holes, difficult to form a single piece, low yield, etc., and achieve the effect of good promotion prospects

Active Publication Date: 2017-07-14
LISHUI AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the technical problems that the black fungus strains used in the existing production have small holes that are not easy to

Method used

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  • Strain of auricularia auricula
  • Strain of auricularia auricula
  • Strain of auricularia auricula

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Select local black fungus "Xinke" and "916" as hybrid parents, and obtain F1 generation hybrid strains through single-single hybridization and single-double hybridization techniques. After laboratory mycelium growth observation and cultivation tests, the strains were screened out. For strains with vigorous silk growth, solitary fruiting bodies, and higher yield than the parent, the fruiting bodies are collected to obtain the second-generation strains through tissue separation technology; further cultivation experiments are carried out to screen and obtain excellent strains with relatively stable target agronomic traits, and hybrid strains are obtained after tissue separation The number "X6" was screened and entered into the cultivation test. This strain has good early maturity, solitary fruiting bodies, and less back tendons. Afterwards, a stable strain was obtained after single-plant screening. This strain improved the shortcomings of the old variety "Xinke",...

Embodiment 2

[0044] Example 2: RAPD method was used to compare the DNA differences between the strain and its parent.

[0045] Detection basis: According to the agricultural industry standard of the People's Republic of China "NY / T 1743-2009 Authenticity Identification of Edible Fungus Strains - RAPD Method", the strain DNA was extracted and the optimal primer was determined.

[0046] Materials submitted for inspection: 3 black fungus strains, among which the strain to be inspected is Lier No. 3, and 2 control strains are "Xinke" and "916".

[0047] Inspection method: The experimental primer sequence (Table 6) was synthesized by Qingke Biotechnology Co., Ltd. in accordance with "NY / T 1743-2009 RAPD method for authenticity identification of edible fungus strains".

[0048] The mycelium activation, cultivation, genomic DNA extraction, quality inspection, PCR amplification and electrophoresis detection of the strains to be tested are all in accordance with the Agricultural Industry Standard o...

Embodiment 3

[0053] Example 3: Comparison of DNA differences between the bacterial strain and its parent using the antagonistic reaction method.

[0054] Test basis: Inoculation, culture and observation of the samples to be tested are carried out according to the Agricultural Industry Standard of the People's Republic of China "NY / T 1845-2010 Differential Identification of Edible Fungi Species Antagonism Reaction".

[0055] Materials submitted for inspection: 3 black fungus strains, among which the strain to be inspected is Lier No. 3, and 2 control strains are "Xinke" and "916".

[0056] Inspection method: Strictly follow the aseptic operation, use a Φ5 mm hole punch to punch out the edge of the colony of the appropriate age after activation as the inoculation block, and place the two inoculation blocks at a distance of 30 mm, and place them at a distance of 15 mm from the center of the PDA plate. Wire up. At a temperature of 25°C, ventilated and protected from light, cultured until the ...

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Abstract

The invention discloses a strain of auricularia auricula, which is named Li'er No.3 and belongs to the genera of auricularia auricula. The strain is preserved in China General Microbiological Culture Collection Center, wherein the preservation address is Chaoyang District of Beijing, Beichen West Rd., 1st yard, No.3, at Jul 5th, 2016, and the accession number is CGMCC No. 12525. The Li'er No.3 strain has the characters of solitary single-sheet fruiting body, moderate lug size, being free of root, being bowl-like or ear-like in shape, being grey in color of back side and black in abdomen surface when dried, being glossy, having significant color difference between the front and the back side, having round and complete edge of the lugs, and having non-obvious ribs and collaterals on the back side, and the like. The single sheet rate during growth of the auricularia auricular reaches more than 90%. Defects of poor resistance of "Xin Ke" and poor lug shapes in "916" are improved. The strain of auricularia auricula has excellent promotion prospect.

Description

technical field [0001] The invention relates to a black fungus strain. Background technique [0002] Black fungus [Auricularia auricula (Lex Hook) Underw] belongs to the Basidiomycota, Lex Hook, Auricularia, Auriculariaceae, and Auricularia genus. It is the first higher colloidal basidiomycete that began to be artificially cultivated in the world. It has moisturizing, strong, and lung-clearing benefits It has the effects of nourishing qi, nourishing blood and promoting blood circulation, and is deeply loved by people. Lishui City, Zhejiang Province is the main planting area of ​​black fungus in southern China. In recent years, the cultivation scale has stabilized at about 150 million sticks. With the expansion of the cultivation scale of black fungus substitutes, different standards have emerged for the classification of black fungus in the market. Features such as dark brown, small ear pieces, single piece, and thick flesh have become more and more evaluation criteria for ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/145C12R2001/645
Inventor 宋小亚刘昆郑巧平李阳路新彦
Owner LISHUI AGRI SCI
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