Identification method of phylogenetic relationship of pepper tree

A technology of kinship and identification method, which is applied in the field of phylogenetic identification of gall pepper tree and detection of nursery stock breeding, achieving the effects of high polymorphism, clear bands and low cost

Inactive Publication Date: 2020-06-23
NORTHWEST UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

Genetic relationship identification and genetic diversity analysis using SRAP molecular markers has not been reported yet

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  • Identification method of phylogenetic relationship of pepper tree
  • Identification method of phylogenetic relationship of pepper tree
  • Identification method of phylogenetic relationship of pepper tree

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Embodiment 1

[0028] The present embodiment provides a kind of method for identification of paprika phylogenetic relationship, and the specific process is as follows:

[0029] (1) Extract male genomic DNA and hermaphrodite genomic DNA from different populations of P. japonica, and construct DNA pools of different populations and different sexes respectively;

[0030] DNA extraction: dry and preserve the young leaves of Chili pepper tree collected from seven different populations of ten male samples and eleven hermaphrodite samples (except for SNB, the hermaphrodite fruits of the other samples develop in the second year after fertilization) , see Table 1 for the specific sampling point numbers, plot names, and collection quantities.

[0031] Table 1 Collection information

[0032]

[0033] Using TIANGEN's Plant Genomic DNA Kit, the genomic DNA was extracted from the young leaves of the male and hermaphroditic plants of the above 11 groups. DNA was extracted from 5 male plants and 5 herma...

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Abstract

The invention discloses a method for identifying the genetic relationship of the gall pepper tree. The sample materials of different populations are subjected to PCR amplification, polyacrylamide gel electrophoresis, silver staining and color development, taking photos and recording the results. The obtained [0,1 ] The data matrix table was analyzed using genetic analysis software, and the genetic relationship between different populations was accurately analyzed, a genetic relationship dendrogram was constructed, and different populations were distinguished. At the same time, this method was used to study the genetic evolutionary relationship between different populations and different genders, and it was speculated that the current androdioecious breeding system of gall pepper trees was derived from the original hermaphroditism. The invention has stable reaction, clear amplification fragments and high polymorphism, can accurately and quickly analyze the genetic relationship and genetic diversity among different populations of gall pepper trees, and can quickly and accurately perform cluster analysis to distinguish different populations.

Description

technical field [0001] The invention belongs to the technical field of nursery stock breeding, and relates to a detection method for seedling breeding, in particular to a method for identifying the kinship relationship of gall pepper trees. Background technique [0002] Gall pepper tree (Tapiscia sinensis Oliv) belongs to the family Tapiscia sinensis and is a perennial deciduous tree. Male plants and hermaphroditic plants in the population are dioecious (androgenic dioecious plants), and the male plants do not bear fruit. Hermaphrodite plants complete fertilization in early June, The latter fruit enters a dormant state and does not start to develop until March of the second year, and hermaphrodite flowers in natural populations can produce fertile pollen. Gall pepper tree is an ancient Tertiary paleotropical relic tree species unique to my country, and a national third-level protected plant. As an ancient and endangered tree species, it has important theoretical significanc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 任小龙刘文哲贾国伦杨柯辛桂亮刘惠东
Owner NORTHWEST UNIV
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