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Method for improving rooting efficiency of muskmelon seedlings

A muskmelon, high-efficiency technology, applied in the field of improving the rooting efficiency of muskmelon seedlings, can solve the problems of low reproduction efficiency, and achieve the effects of good market prospects, good economic benefits, and easy operation

Inactive Publication Date: 2017-08-08
李茂兰 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, cantaloupe is mainly propagated by seeds, and the reproduction efficiency is low.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for improving the rooting efficiency of muskmelon seedlings, soaking the seeds of muskmelon in a potassium permanganate solution with a mass concentration of 1%, and then rinsing with sterile water for 3 minutes to obtain sterile seeds; inoculating the sterile seeds into In the primary medium, the primary medium is MS + sucrose 20 g / L for primary culture, 20°C, humidity 60%, light 2000lx, to obtain sterile seedlings; the cotyledons and hypocotyls of the sterile seedlings are used as explants body, and inoculated in the subculture medium, the subculture medium is MS + 6-benzylaminoadenine 0.05 mg / L + naphthaleneacetic acid 0.1 mg / L + sucrose 20 g / L, 25°C, humidity 70%, light 6000 lx, carry out subculture, obtain rootless seedling; Transplant described rootless seedling in rooting medium, rooting medium is 1 / 2MS macroelement+MS trace element+MS iron salt+MS organic matter+naphthalene acetic acid 3 mg / L+sucrose 20 g / L, 28 ℃, humidity 80%, light 10000 lx, carry ou...

Embodiment 2

[0022] A method for improving the rooting efficiency of muskmelon seedlings, soaking the seeds of muskmelon in a potassium permanganate solution with a mass concentration of 3%, and then rinsing with sterile water for 5 minutes to obtain aseptic seeds; inoculating the aseptic seeds into In the primary medium, the primary medium is MS + sucrose 20 g / L for primary culture, 22°C, humidity 70%, light 5000 lx, to obtain sterile seedlings; the cotyledons and hypocotyls of the sterile seedlings were used as Plants were planted and inoculated in the subculture medium, which was MS + 6-benzylaminoadenine 0.05 mg / L + naphthaleneacetic acid 0.1 mg / L + sucrose 20 g / L, at 28°C, humidity 80%, Illuminate 8000 lx, carry out subculture, obtain rootless seedling; Transplant described rootless seedling in rooting medium, rooting medium is 1 / 2MS macroelement+MS trace element+MS iron salt+MS organic matter+naphthaleneacetic acid 3 mg / L+sucrose 20 g / L, 30°C, humidity 90%, light 15000lx, for rooting...

Embodiment 3

[0024] A method for improving the rooting efficiency of muskmelon seedlings, soaking the seeds of muskmelon in a potassium permanganate solution with a mass concentration of 2%, and then washing them with sterile water for 4 minutes to obtain aseptic seeds; inoculating the aseptic seeds into In the primary medium, the primary medium is MS + sucrose 20 g / L for primary culture at 25°C, humidity 70%, and light 3000 lx to obtain sterile seedlings; the cotyledons and hypocotyls of the sterile seedlings are used as explanted body, and inoculated in the subculture medium, the subculture medium was MS + 6-benzylaminoadenine 0.05 mg / L + naphthaleneacetic acid 0.1 mg / L + sucrose 20 g / L, 28°C, humidity 75%, light 7000 lx, carry out subculture, obtain rootless seedling; Transplant described rootless seedling in rooting medium, rooting medium is 1 / 2MS macroelement+MS trace element+MS iron salt+MS organic matter+naphthalene acetic acid 3 mg / L+sucrose 20 g / L, 28°C, humidity 85%, light 12000...

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Abstract

The invention discloses a method for improving the rooting efficiency of muskmelon seedlings. The method comprises the steps of soaking muskmelon seeds in a potassium permanganate solution of which the mass concentration is 1 to 3 percent, and then washing the muskmelon seeds by using sterile water to obtain sterile seeds; inoculating the sterile seeds into a primary culture medium for primary culture to obtain sterile seedlings; taking cotyledons and hypocotyledonary axis of the sterile seedlings as explants, inoculating the sterile seedlings into a secondary culture medium for secondary culture to obtain rootless seedlings; transplanting the rootless seedlings into a rooting culture medium for rooting culture to obtain muskmelon seedlings. A tissue culture seedling growth method has the advantages of high rooting efficiency and high survival rate, and good quality of a plant can be maintained.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture and seedling cultivation, and in particular relates to a method for improving the rooting efficiency of muskmelon seedlings. Background technique [0002] Cantaloupe contains a lot of carbohydrates and citric acid, etc., and has plenty of water, which can relieve summer heat, promote body fluids, quench thirst, and relieve troubles; the invertase in cantaloupe can convert insoluble protein into soluble protein, which can help kidney patients absorb nutrients; the vitamins in cantaloupe stalk B can protect the liver and reduce chronic liver damage. According to the "Compendium of Materia Medica" records: cantaloupe, there are many kinds of melons in the northern soil and Zhongzhou. Planted in February and March, spread and grow, leaves are several inches large, yellow flowers bloom in May and June, and melons are ripe in June and July. There are many kinds of them, some are clusters, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李茂兰蒋军
Owner 李茂兰
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