Bio-compound fertilizer and preparation method thereof
A bio-composite fertilizer and blue-green algae technology, applied to organic fertilizers, fertilization devices, fertilizer mixtures, etc., can solve the problems of complex preparation process, limited production increase, long production cycle, etc., to achieve fast growth cycle, strong vitality, and increase production. Effect
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[0048] Example 1
[0049] Prepare biological compound fertilizer as follows:
[0050] 1. Cultivation of nitrogen-fixing Anabaena: (1) Pick a small ring of nitrogen-fixing Anabaena seed, inoculate it on a BG-11 solid medium plate, streak and separate, and cultivate under the conditions of 28° C. and 3000 lux of light. (2) Pick a single algal colony from the activated BG-11 plate, inoculate it into 100 mL of BG-11 liquid medium, and cultivate it continuously for 5 days on a constant temperature shaker at 28°C, light 3000 lux, and 140 r / m. (3) The nitrogen-fixing Anabaena was inoculated into 50L BG-11 liquid medium according to the inoculation amount of 7% (v / v), under the conditions of 28° C., light of 300lux, light-dark ratio of 12h: 12h and 140r / m Cultivate nitrogen-fixing Anabaena for 7 to 8 days until its concentration reaches 10 6 ~10 7 pcs / mL.
[0051] 2. Cultivate Chlorella pyrenoidosa: (1) Pick a small ring of Chlorella pyrenoidosa algal species, streak on the steril...
Example Embodiment
[0053] Example 2
[0054] The biological compound fertilizer was prepared with reference to the method described in Example 1, except that the nitrogen-fixing Anabaena and Chlorella pyrenoidosa were mixed according to the following weight parts: 40 parts of nitrogen-fixing Anabaena and 7 parts of Chlorella pyrenoidea.
Example Embodiment
[0055] Example 3
[0056] The biological compound fertilizer was prepared with reference to the method described in Example 1, except that the nitrogen-fixing Anabaena and Chlorella pyrenoidosa were mixed according to the following weight parts: 45 parts of nitrogen-fixing Anabaena and 10 parts of Chlorella pyrenoidea.
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