Bio-compound fertilizer and preparation method thereof
A bio-composite fertilizer and blue-green algae technology, applied to organic fertilizers, fertilization devices, fertilizer mixtures, etc., can solve the problems of complex preparation process, limited production increase, long production cycle, etc., to achieve fast growth cycle, strong vitality, and increase production. Effect
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Embodiment 1
[0049] Prepare biological compound fertilizer as follows:
[0050] 1. Cultivation of nitrogen-fixing Anabaena: (1) Pick a small circle of nitrogen-fixing Anabaena species, inoculate it on a BG-11 solid medium plate, streak and separate it, and cultivate it under the conditions of 28°C and 3000 lux light. (2) Pick a single algae colony from the activated BG-11 plate, inoculate it into 100 mL of BG-11 liquid medium, and culture continuously for 5 days on a constant temperature shaker at 28°C, 3000 lux light, and 140 r / m. (3) Inoculate nitrogen-fixing Anabaena into 50L BG-11 liquid medium at an inoculum size of 7% (v / v), under the conditions of 28°C, 300lux light, 12h:12h light-dark ratio and 140r / m Cultivate nitrogen-fixing Anabaena for 7-8 days until its concentration reaches 10 6 ~10 7 individual / mL.
[0051] 2. Cultivate Chlorella pyrenoidosa: (1) Pick a small circle of Chlorella pyrenoidosa species, draw a line on the sterilized Basal solid medium plate containing 10g / L g...
Embodiment 2
[0054] The biological compound fertilizer was prepared with reference to the method described in Example 1, the only difference being that nitrogen-fixing Anabaena and Chlorella pyrenoidosa were mixed in the following parts by weight: 40 parts of nitrogen-fixing Anabaena pyrenoidosa and 7 parts of Chlorella pyrenoidosa.
Embodiment 3
[0056] The biological compound fertilizer was prepared with reference to the method described in Example 1, the only difference being that nitrogen-fixing Anabaena and Chlorella pyrenoidosa were mixed in the following parts by weight: 45 parts of nitrogen-fixing Anabaena pyrenoidosa and 10 parts of Chlorella pyrenoidosa.
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