Specific primer for quantitatively detecting yellow leaf curl virus, as well as application of specific primer
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- BEIJING PLANT PROTECTION STATION
- Publication Date
- 2017-08-18
- Estimated Expiration
- Not applicable · inactive patent
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Abstract
Description
technical field
[0001] The invention belongs to the field of pathogen detection, in particular to a specific primer for quantitative detection of yellow leaf curl virus and its application. Background technique
[0002] Traditional detection methods of pathogenic bacteria, such as physiological and biochemical index identification methods, bacterial isolation and culture identification methods, etc., rely on phenotypes to identify pathogenic bacteria; these methods are time-consuming, have low sensitivity, and poor accuracy.
[0003] Propidium azide bromide (PMA) is a fluorescent dye with high affinity to DNA, which can pass through the damaged cell membrane and covalently cross-link with DNA under the irradiation of visible light, so as to block the DNA in damaged cells. The role of DNA for PCR amplification. Some studies have reported that the PMA-qPCR method combined with the detection of live pathogen content, but only applied in water (Fujimoto J, Watanabe K. Quantitat...