PDMS material-based microchamber array type digital PCR chip

Abstract

The invention relates to a PDMS material-based microchamber array type digital PCR chip. The chip is made of a PDMS material and comprises a sample inlet hole, a microchamber array and a sample outlet hole; the sample inlet hole comprises a sample inlet and a water sample inlet; the sample outlet hole comprises a sample outlet and a water sample outlet; the water sample inlet and the water sample outlet are communicated with the microchamber array at the periphery of the chip; and the sample inlet and the sample outlet are communicated with each reaction chamber in the microchamber array through a microchannel. According to the PDMS material-based microchamber array type digital PCR chip, water channels at the periphery of the chip can prevent PCR reaction liquid in the PCR process from evaporating; a designed automatic counting software can automatically counts a chip result; and the PDMS material-based microchamber array type digital PCR chip can reach very high detection sensitivity and realizes single molecule detection of DNA.

Description

technical field [0001] The invention belongs to the field of digital PCR, in particular to a microchamber array digital PCR chip based on PDMS material. Background technique [0002] Digital PCR is a technique capable of absolute quantification of the copy number of DNA molecules. This technology has many advantages: it can accurately quantify the starting sample, and does not need to draw a standard curve in advance; it has high sensitivity, and the detection limit can reach a single molecule; it can quantify small concentration changes of nucleic acid molecules, etc. These advantages make digital PCR more accurate and sensitive than traditional quantitative methods, so it is more suitable for the quantitative detection of DNA molecules. [0003] Digital PCR chips have also been one of the research hotspots in the field of microfluidic chips. At present, digital PCR microfluidic systems are mainly divided into two types: emulsion droplet and array. The first is the emuls...

AI Technical Summary

Problems solved by technology

[0005] At present, emulsion digital PCR requires complex emulsion generation, manipulation, and readout equipment, so commercial instruments based on emulsion digital PCR are very expensive

However, the current array digital PCR is still immature, or the structure is complex or the operation is cumbersome.

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