A monochromatic ECL immunoassay method based on green light radiation quantum dots

Abstract

The invention discloses a monochromatic ECL immunodetection method based on green light radiation quantum dots, which mainly includes (1) preparation of CdSe quantum dot-labeled secondary antibody (CdSe QDs-Ab2), (2) using CdSe quantum dots as markers The preparation of the single-color ECL immunosensor and (3) drawing the working curve, carry out three steps of single-color ECL immunodetection. The detection sensitivity of the method of the invention is high, the detection limit reaches 0.1fg / mL, and single-molecule detection of the antigen can be realized; the selectivity is high. The electrochemiluminescent immunosensor constructed in the present invention is based on the specific recognition and binding between the antigen and the antibody, so the interfering protein in the test solution cannot combine with the first antibody and the second antibody of the antigen. No distractions.

Description

technical field

[0001] The present invention relates to an electrogenerated chemiluminescence (electrogenerated chemiluminescence, ECL) immunoassay method, in particular to an immunoassay using highly passivated CdSe quantum dots as markers on the surface that can produce monochromatic electrochemiluminescence radiation in the green light region. Detection method. Background technique

[0002] At present, most ECL kits and clinical diagnostic techniques use Ru(bpy) 3 2+ as an ECL marker. Given Ru(bpy) 3 2+ The ECL radiation spectrum of the system is wide, and it is located in the red light region. The development of an efficient monochromatic ECL system in the non-red light region is of great value for the basic and applied research of ECL analysis.

[0003] Quantum dots are a new type of ECL emitter, and their unique photoelectric properties provide the possibility for the development of ECL systems in non-red regions. Liang et al. developed a near-infrared ECL immuno...

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