Modified activated carbon carrier for immobilizing phorate degradation bacterium and preparation method thereof
The technology of activated carbon and degrading bacteria is applied in the field of modified activated carbon carrier for immobilizing phorate degrading bacteria and its preparation field. high effect
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[0039] (1) 27.8g of activated carbon is added to 300mL of NaHCO with a mass percent concentration of 15%. 3 solution, stirred at 1000r / min for 50 minutes, filtered to remove the liquid, washed with 500mL of deionized water and placed in a drying oven at 102°C for 40 minutes to obtain substance A;
[0040] (2) Add 12.9 grams of 3-chloro-6-phenylpyridazine to 800 mL of ethanol, shake in a shaker at a temperature of 35°C for 35 minutes, divide into 4 equal parts after shaking well, and obtain the mixed solution G1, mix Liquid G2, mixed liquid G3, mixed liquid G4;
[0041] (3) 7.5 grams of 4-(2-aminoethyl)tetrahydropyran and 8.9 grams of 2-bromo-5-cyanopyrazine were added to 200 mL of chloroform and shaken in a shaker at a temperature of 35°C After 35 minutes, shake well and divide into 4 equal parts to obtain mixed solution H1, mixed solution H2, mixed solution H3, and mixed solution H4;
[0042] (4) Add mixed solution H1 and 8.8 g of 5-chloro-3-methylbenzothiophene to mixed so...
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