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Bacillus pumilus rp01 and its application

A technology of Bacillus pumilus and bacterial agents, applied in the field of bio-agricultural science and technology, can solve the problems of poor ecological adaptability and inability to achieve crop growth-promoting effects, and achieve the goals of improving phosphorus nutrition, high inorganic phosphorus solubility, and promoting plant growth Effect

Active Publication Date: 2020-04-28
重庆千明农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the relevant researches are based on sterilizing the soil or matrix, and then carrying out research on the effects of inoculating PGPR on plant growth and other effects. Poor sex and other problems, unable to compete with indigenous microorganisms and "annihilate", unable to achieve the growth-promoting effect on crops

Method used

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  • Bacillus pumilus rp01 and its application
  • Bacillus pumilus rp01 and its application
  • Bacillus pumilus rp01 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Screening and Identification of Bacillus pumilus RP01

[0023] 1. Screening of Bacillus pumilus RP01

[0024] 1. Material preparation

[0025] (1) Test soil

[0026] The soil was collected from the vegetable rotation (lettuce-pepper-soybean-pakchoi) at the National Purple Soil Fertility and Fertilizer Effect Monitoring Base of Southwest University in Beibei District, Chongqing City. High planting area. Select 3 pieces of soil with a range of 1 × 1m, randomly collect 10-20cm soil according to the 5-point sampling method, mix the soil samples evenly, put them in an ice bucket, and bring them back to the laboratory and store them in a refrigerator at 4°C for later use. The basic physical and chemical properties of the soil are listed in Table 1.

[0027] Table 1 Basic physical and chemical properties of the tested soil

[0028]

[0029] (2) Configure culture medium

[0030] Phospholytic bacteria selection medium: NaCl 0.2g; MgSO 4 ·7H 2 O 0.1g; MnSO 4 4H 2 O 0...

Embodiment 2

[0058] Determination of Phosphorus Solubilizing Ability of Bacillus pumilus RP01

[0059] Referring to the method in the literature (Yu Weibin, 2010), prepare LB liquid culture medium, take 100mL and divide into 250mL Erlenmeyer flasks. After autoclaving, inoculate RP01, culture at 28°C and 120r / min for 24 hours, and dilute the bacterial concentration of the fermentation liquid to 10 8 cfu / mL, spare.

[0060] Prepare phosphate-dissolving bacteria selective liquid medium, take 100mL and divide it into 250mL Erlenmeyer flasks, and after autoclaving at 121°C for 30min, the test group was inoculated with 2mL of the above bacteria with a concentration of 10 8 cfu / mL fermentation broth, the control group was added 2 mL of high-temperature sterilized LB liquid medium, and each treatment was repeated three times. All were cultured at 28°C and 120r / min for 7 days. Use the diluted plate colony counting method (phosphorus-dissolving bacteria selective medium, cultivated at 28°C for 3 ...

Embodiment 3

[0066] Determination of Growth-promoting Ability of Bacillus pumilus RP01 on Tobacco Seedlings

[0067] After the petri dish is sterilized, cover the filter paper, moisten it with distilled water, evenly spread the K326 tobacco seeds on the filter paper, and accelerate germination at 25±1°C for 3-4d (the filter paper of the petri dish should be kept moist during this period). The tobacco seeds after the germination are transferred to the seedling trays equipped with substrate (60% humus soil+40% perlite), and 2 tobacco seeds through the germination are put in each hole, which are covered with substrate, and each hole is reserved after emergence. A tobacco seedling.

[0068] When the tobacco grows to 1 heart of 3-4 leaves, start to inoculate the RP01 bacterial liquid (the bacterial liquid is prepared according to the method in Example 2). Set 3 treatments: control, bacterial solution concentration 10 3 cfu / mL and 10 6 cfu / mL two test groups. Repeat four times, inoculate 1mL...

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Abstract

The present invention relates to Bacillus pumilus (Bacillus pumilus) RP01 and its application. The strain has the function of dissolving inorganic phosphorus and can effectively promote plant growth. It is determined by experiments that on the PKO inorganic phosphorus medium, when the bacterial concentration is 1×10 7 When the cfu / ml was cultured at 28°C and 120r / min for 7 days, the dissolved phosphorus content of the fermentation liquid was as high as 129.0±11.89mg / L, showing a high inorganic phosphorus dissolving ability. It can be used to prepare microbial fertilizer bacterial agents, which can effectively improve the phosphorus nutrition of crops in the acidic soil in southern my country, thereby reducing the use of chemical fertilizers.

Description

technical field [0001] The invention belongs to the application field of bio-agricultural science and technology, and in particular relates to Bacillus pumilus RP01 and an application thereof. Background technique [0002] In 1904, German microbiologists first proposed the concept of plant rhizosphere (rhizosphere), which refers to the area of ​​several millimeters around plant roots. The plant rhizosphere is a microenvironment at the interface between the plant root system and the soil, and it is the interaction site where the soil, root system and microorganisms are closely combined. The rhizosphere microbial population is mainly composed of bacteria. According to their different effects on plants, rhizobacteria can be roughly divided into beneficial bacteria (2%-5%), harmful bacteria (8%-15%) and Neutral bacteria (80%-90%) three categories. Plant growth-promoting rhizobacteria (PGPR) refers to a group of bacteria that can live freely in the soil or colonize in the rhizo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P21/00C05F11/08C12R1/07
CPCA01N63/00C05F11/08C12N1/205C12R2001/07
Inventor 杨星勇申鸿华人杰秦利均
Owner 重庆千明农业发展有限公司