Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for detecting milk protein and milk fat synthesis ability of cow mammary glands by using cow's milk

A milk protein and milk fat technology is applied in the field of detecting the ability of the mammary gland to synthesize milk protein and milk fat, and can solve the problem of not being able to accurately and comprehensively reflect the ability of the mammary gland to synthesize milk protein and milk fat.

Inactive Publication Date: 2017-10-20
NORTHEAST AGRICULTURAL UNIVERSITY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the method for detecting milk protein and milk fat of raw milk at this stage cannot accurately and comprehensively reflect the ability of different dairy cow breeds and individual mammary glands to synthesize milk protein and milk fat, and provides a method for detecting milk protein and milk fat using milk. Method for Synthesizing Milk Protein and Milk Fat Ability of Dairy Cow Mammary Gland

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting milk protein and milk fat synthesis ability of cow mammary glands by using cow's milk
  • Method for detecting milk protein and milk fat synthesis ability of cow mammary glands by using cow's milk
  • Method for detecting milk protein and milk fat synthesis ability of cow mammary glands by using cow's milk

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0013] Specific Embodiment 1: In this embodiment, a method for detecting milk protein and milk fat synthesis ability of cow mammary glands using cow milk: 1. Pretreatment of cow milk: take cow milk and freeze it at -80°C for later use to obtain milk samples; 2. Preparation of secretions: centrifuge milk samples at 0-4°C, 2000-2500rpm for 25-30min, remove precipitated cells and floating fat globules, and obtain skim milk; centrifuge skim milk at 0-4°C, 12000- Centrifuge at 13000rpm for 30-40min and take the supernatant; centrifuge the supernatant at 0-4°C and 100000-110000rpm for 2-2.5h to take the precipitate; dissolve the precipitate with 0.01-0.02M PBS to obtain the outer Exosome primary solution; exosome primary solution at 0-4°C,

[0014] Centrifuge at 100000-110000rpm for 2-2.5h, take the precipitate, dissolve the precipitate with 0.01-0.02M PBS solution, obtain the exosome solution, test the purity of the exosome solution, and proceed to the next step after passing the t...

specific Embodiment approach 2

[0027] Embodiment 2: This embodiment differs from Embodiment 1 in that: Western blotting is used to detect the purity of the exosome solution. Others are the same as the first embodiment.

[0028] In this embodiment, the steps for detecting the purity of the exosome solution by Western blotting are:

[0029] 1. Sample preparation: transfer the exosome solution to a 1.5ml centrifuge tube, add 2× loading buffer or 5× loading buffer, and then place in a boiling water bath for 10 minutes; ultrasonicate the cell lysate 3 times, 15s each time; subpackage, and store in a -80°C ultra-low temperature refrigerator for later use;

[0030] 2. Preparation of separation gel: prepare 10% separation gel solution, add TEMED to the separation gel solution, mix well and inject between two glass plates, use 7ml of glue, add water with a 200ul pipette gun to isolate the air, and make The glue surface is smooth, after standing for 20 minutes, pour off the upper layer of water, and dry it with fil...

specific Embodiment approach 3

[0038] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the proteins used for the detection of exosome purity include: TSG101, GRP78, vWF and GAPDH. Others are different from the first or second specific embodiment.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a method for detecting the milk protein and milk fat synthesis ability of cow mammary glands by using cow's milk. The purpose of the invention is to solve a problem that the milk protein and milk fat synthesis ability of mammary glands of different species and individuals of cows cannot be accurately and comprehensively reflected through existing methods for detecting the milk protein percentage and the milk fat percentage of raw milk. The method comprises the following steps: 1, preprocessing the cow's milk; 2, preparing a cow's milk exosome; 3, detecting the GlyRS content and the CAP1 content; and 4, dividing the GlyRS content by the CAP1 content to obtain the milk protein and milk fat synthesis ability of cow mammary glands. The value of the GlyRS content / the CAP1 content can reflect the milk protein and milk fat synthesis ability of the cow mammary glands, and also can solve the problem of ineffective judgment of the mammary gland's synthesis ability due to the influences of other factors on the milk protein percentage and the milk fat percentage. The method can be applied to the feed of cow raising.

Description

technical field [0001] The invention relates to a method for detecting milk protein and milk fat synthesis ability of milk cow mammary gland by using milk. Background technique [0002] Milk quality mainly refers to milk protein rate and milk fat rate, which are important economic indicators of milk. High-quality milk has higher milk protein and milk fat percentages, and has better dairy processing characteristics, so the market price is also higher. Under the same feeding and nutrition environment, the milk protein and milk fat percentages of raw milk produced by different breeds of cows and different individuals of the same breed of cows are quite different. An important indicator of differences in milk production quality between individual mammary glands. The ability of the mammary gland to synthesize milk protein and milk fat is determined by the key genes that regulate the synthesis of milk protein and milk fat in the mammary gland epithelial cells, the basic unit of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/04
CPCG01N33/04
Inventor 高学军郭思圻张明辉刘营敖金霞曲波袁肖寒
Owner NORTHEAST AGRICULTURAL UNIVERSITY