Permanent cell line for ecthyreosis virus multiplication and establishment method of permanent cell line

Abstract

The invention relates to a permanent cell line for ecthyreosis virus multiplication. The name of the cell line is a goat testis cell GT26, the cell line is preserved at the China Center for Type Culture Collection on March 1, 2017, and the preservation number is CCTCC NO:C201734. An establishment method of the permanent cell line comprises the steps of (1) permanent establishment of the goat testis cell; (2) low-serum domestication of the goat testis cell; and (3) cultivation of an ecthyreosis virus in a newborn goat testis cell line. The ecthyreosis virus is grafted by using the goat testis cell line, thereby achieving the target of stable virus multiplication and providing a stable cell environment and a standardized culture method for development and production of an attenuated vaccine and an inactivated vaccine of the ecthyreosis virus.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to a permanent cell line, in particular to a permanent cell line for the multiplication of aphthous ulcer virus and its culture method and application. Background technique [0002] Sheep contagious impetigo, also known as Orf virus, is a zoonotic infectious disease caused by Orf virus. The disease is distributed in almost all sheep-raising countries in the world, and mainly affects sheep and goats under natural conditions, especially goats are the most prone. The prevalence of this disease has no obvious seasonality, but it is relatively easy to occur in spring and autumn. It is often a mass epidemic, and lambs aged 3 to 6 months are most susceptible to infection. Sick sheep and infected sheep are the source of infection of the disease. Healthy sheep are infected by direct contact with sick sheep, or by contact with utensils, drinking water, feeding troughs, feed, etc. contaminated by s...

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Problems solved by technology

[0004] But there is no oral ulcer vaccine available for users in the market at present, the main reason is that there is no practical cell line for the production of oral oral ulcer vaccine, and primary cells such as bovine testicular primary cells, sheep fetal turbinate cells, and sheep fetal skin fibroblasts are prepared There are many problems in the process: such as the limited number of samples, complicated preparation process, high cost, long time-consuming, unstable quality between batches, etc., which makes it difficult for enterprises to achieve large-scale production; at the same time, it can be used to inoculate the cell line of canker sores (MDBK) The titer of proliferating virus is not high

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