Preparation method of citrinin compound dicitrinone D and its application in lymphoma

A compound, the technology of citrinin, applied in the field of preparation of citrinin compound dicitrinoneD

Inactive Publication Date: 2019-07-09
FUZHOU UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that the citrinin compounds shown have anti-lymphoma activity, and there is no report on the proliferation inhibitory activity of this compound on human lymphoma cell U937, so there is no drug related to this in the market.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of citrinin compound dicitrinone D and its application in lymphoma
  • Preparation method of citrinin compound dicitrinone D and its application in lymphoma
  • Preparation method of citrinin compound dicitrinone D and its application in lymphoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Fermentation production and separation and purification of the compound

[0022] 1 Fermentation production

[0023] Fermentation culture of producing bacteria: According to the conventional method of cultivating microorganisms, take Penicillium citrinum IBPT-5 (preserved in the China Type Culture Collection on December 25, 2013, address: Wuhan University, Wuhan, the deposit number is : CCTCC NO: M 2013713) an appropriate amount, inoculate on the PDA solid slant medium and culture in a 28°C incubator for 4 days.

[0024] Take an appropriate amount of Penicillium citrinum IBPT-5 cultured on the slope for 4 days and inoculate it into 400mL of culture solution [Composition of culture solution (g / L): mannitol 20.0, yeast extract 3.0, maltose 20.0, monosodium glutamate 10.0, glucose 10.0, KH 2 PO 4 0.5, MgSO 4 0.3, NaCl 30.0 constant volume] 1000mL Erlenmeyer flask, 28 ℃ static culture for 30 days, obtain mycelium and fermentation broth.

[0025] 2 Obtaining extract

[00...

Embodiment 2

[0032] Example 2 In vitro anti-tumor activity test

[0033] 1 Experimental samples and experimental methods

[0034] Preparation of the test sample solution The test sample is the pure compound separated and refined in the above-mentioned implementation 1. Accurately weigh an appropriate amount of sample and prepare a solution of the required concentration with DMSO for activity measurement.

[0035] The subculture of cell line and cell adopts tumor cell line. For tumor cells, use RPMI 1640 medium containing 10% FBS, and pass 5% CO at 37℃. 2 The incubator for relay culture.

[0036] Cell proliferation inhibitory activity test method

[0037] WST-1 method Take logarithmic growth phase lymphoma cells and adjust the cell density to 5×10 per milliliter 4 Cells were seeded in a 96-well cell culture plate at 100 μL per well, and 5% CO was injected at 37℃ 2 Cultivate overnight in the incubator. Aspirate the supernatant, add 100 μL of medium containing the sample, and continue to incubate for...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method of a citrinin compound dicitrinone D and an application of the citrinin compound dicitrinone D in respect of lymph cancer. The citrinin compound dicitrinone D has a structural formula shown in the description. The compound is obtained by culturing penicillium citrinum IBPT-5 through fermentation to obtain a fermented object, and performing separation and purification to the fermented object. It is proved by the experiments that the compound has preferable antitumor activity to human lymph cancer cells U937, and can be used to prepare a lymph cancer cell proliferation inhibition medicine or an antitumor medicine, which are used to research lymph cancer.

Description

Technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a preparation method of citrinin compound dicitrinone D and its application in lymphoma. Background technique [0002] Citrinin is a kind of nitrogenous organic compound produced by biological secondary metabolism. There are many kinds of citrinin in nature, most of which are derived from plants, so it is also called a plant alkali. Citrinin has important physiological effects on humans and animals, including relieving asthma and relieving cough, lowering blood sugar, lowering blood lipids, antibacterial, antitumor, analgesic, etc. Among them, antibacterial and antitumor activities are the most prominent. The natural structure of citrinin is an important source of lead compounds discovered in innovative drug research. There are nearly one hundred citrinin drugs currently used in clinical practice. Studies have found that some marine fungi can produce citrinin with novel structu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & AuthorityPatents(China)
IPC IPC(8): C07D407/06C12P17/16A61P35/00C12R1/80
CPCC07D407/06C12P17/162
Inventor陈立李亚平赵杨杨周彤张其清
OwnerFUZHOU UNIV