Antibody chip capable simultaneously detecting various cell adhesion factors

Abstract

The invention specifically discloses an antibody chip that can detect multiple cell adhesion factors simultaneously, including antibody chip, cell adhesion factor standard substance, cell adhesion factor standard substance mixture, biotin-labeled cell adhesion factor detection antibody, Biotin-labeled cell adhesion factor detection antibody cocktail; fluorescein-labeled streptavidin. The invention realizes that ten cell adhesion factors can be detected at one time, and the sensitivity of the detected cell adhesion factors can reach the ELISA detection sensitivity of a single factor. The antibody chip kit of the invention overcomes the defects of the prior art such as cumbersome operation, single detection index, low sensitivity, etc., and has the advantages of low cost, convenience, sensitivity, accuracy, high throughput, less sample consumption, and can be popularized and scaled in ordinary laboratories. advantages, etc.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to an antibody chip capable of simultaneously detecting multiple cell adhesion factors. Background technique [0002] Cell adhesion molecules (CAM) are glycoproteins located on the cell surface or in the cell matrix, and function in the form of receptors and ligands. , ECM) is a collective term for the molecules that contact and bind with each other. Cell adhesion factors include the immunoglobulin superfamily, integrin family, selectin family and angioadhesin family. Adhesion molecules enable cell-to-cell, cell-to-matrix adhesion, participate in cell recognition, activation and signal transduction, cell proliferation and differentiation, cell extension and movement, immune response, inflammation, coagulation, tumor metastasis, The molecular basis of a series of important physiological and pathological processes such as wound healing. [0003] Commonly used methods f...

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Problems solved by technology

Flow cytometry is not widely used because it requires expensive instruments and specialized operators

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