Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Tissue culture and rapid propagation method of Gleditsia triacaanthos 'sunburst'

A technology for tissue culture rapid propagation and acacia, which is applied in the field of plant breeding, can solve the problems of easy breakage at the grafting site, there is no report on the tissue culture rapid propagation technology of American golden leaf acacia, and the stress resistance is not strong, and the occurrence rate of insect pests is low. , Not easy to viral diseases, the effect of improving the survival rate of plants

Inactive Publication Date: 2017-11-17
WEIFANG VOCATIONAL COLLEGE
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current American acacia breeding technology is mostly grafting, but due to the faster growth, the grafting is easy to break, and the stress resistance is not strong; and the American acacia that is propagated by tissue culture not only overcomes the above-mentioned defects, but also reproduces quickly, and the seedlings Virus-free, strong resistance to pests and diseases
[0005] There is no report on the tissue culture and rapid propagation technology of American Acacia acacia in the present technology

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue culture and rapid propagation method of Gleditsia triacaanthos 'sunburst'
  • Tissue culture and rapid propagation method of Gleditsia triacaanthos 'sunburst'
  • Tissue culture and rapid propagation method of Gleditsia triacaanthos 'sunburst'

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 A method for tissue culture and rapid propagation of Acacia acacia

[0029] 1. Material preparation

[0030] The test was carried out in the Tissue Culture Center of Weifang Vocational College in 2015, and the test material was Gleditslatriacanthos 'Sunburst'.

[0031] 2. Selection of explants

[0032] Select growth vigorously outdoors, no damage by diseases and insect pests, the single plant of American acacia acacia with good character performance; Cut the explant with scissors, when cutting the explant, the ambient temperature is 25 ℃, and the humidity is 66%;

[0033] The explant of clipping is: the terminal bud stem section of the exuberant branch on the periphery of the crown top, requiring the terminal bud to be full, and the length of the stem section is 1.0cm.

[0034] 3. Disinfection of explants

[0035] Put the explants in the triangular flask, add 75% alcohol to disinfect for 1 min, and then rinse with water for 3 times, rinse thoroughly;

[00...

Embodiment 2

[0059] Example 2 The selected explant specification on the impact experiment on the survival rate

[0060] Carry out tissue culture propagation according to the method of embodiment 1, only change the explant specification selected in step 2, i.e. the length of terminal bud stem segment, carry out embodiment 2~4, see table 1 specifically:

[0061] Explant specifications selected in table 1

[0062]

[0063] Investigate the survival situation of the tissue culture seedlings of embodiment 1~4;

[0064] The survey results are shown in Table 2;

[0065] Table 2 Survival rate of tissue culture seedlings

[0066]

[0067] Investigation result shows, the tissue culture seedling survival rate of embodiment 3 is the highest, is preferred embodiment; Namely the explant specification that chooses in the step 2, terminal bud stem section length is preferably 1.8cm.

Embodiment 5

[0068] Embodiment 5 The influence of different strong seedling rooting medium on tissue culture seedling

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Lengthaaaaaaaaaa
Lengthaaaaaaaaaa
Lengthaaaaaaaaaa
Login to View More

Abstract

The invention provides a tissue culture and rapid propagation method of Gleditsia triacaanthos 'sunburst'. The method comprises steps as follows: selection of an explant, disinfection of the explant, pretreatment, initiation culture, differentiation culture, culture of tissue culture seedlings, seedling hardening and domestication, wherein the explant is selected as follows: terminal bud stem segments of flourishing branches on the periphery of the upper part of the tree crow are selected as the explant, the terminal buds are required to be plump, and the length of the stem segments is 1-1.8 cm; the explant is sheared with a pair of scissors at the environment temperature of 22-28 DEG C and at the humidity of 65%-70%; pretreatment is performed as follows: a 0.05 mg / L water solution is prepared from gibberellin, 6-BA, IBA and monopotassium phosphate in the ratio of 2:1:2:3, and a pretreatment solution is obtained; the disinfected explant is soaked in the pretreatment solution for 8 hours. With the adoption of the tissue culture and rapid propagation method of Gleditsia triacaanthos 'sunburst', the propagation speed is high, and the plant survival rate is increased by 15.5%.

Description

technical field [0001] The invention relates to a method for breeding acacia acacia, in particular to a method for tissue culture and rapid propagation of acacia acacia, belonging to the technical field of plant breeding. Background technique [0002] ​American honey locust, broad-leaved deciduous tree, plant height 9-10.5 meters, fast growing, no branch thorns, young leaves golden, mature leaves of honey locust are light yellow-green, and are still light yellow-green in autumn, branches stretch, plant type Beautiful, not strong. [0003] It is light-loving and slightly shade-tolerant, drought-tolerant, cold-resistant, and can withstand low temperatures of -34 degrees. It likes warm and humid climate and deep fertile soil. It can also grow on calcareous and mild saline-alkali soil. It has wide adaptability and can be planted from northern to southern and southwest China. Deep roots, less pests and diseases. During production, it was found that plants with a DBH below 3 cm...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 丁世民吴祥春孙曰波赵从凯杨兴芳李寿冰郭明明郭继民陈大雷
Owner WEIFANG VOCATIONAL COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com