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Compound, preparation method and application thereof and product containing compound

A technology for compounds and products, applied in the fields of botanical equipment and methods, microorganism-based methods, biochemical equipment and methods, etc., which can solve problems such as crop production loss, achieve high practicability, regulate plant stress resistance, and improve resistance. The effect of adversity coercion ability

Inactive Publication Date: 2017-11-24
内蒙古康元生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the change of global climate, high temperature, low temperature, drought and waterlogging will occur alternately and frequently, and the environmental pressure caused by soil salinization, heavy metal pollution, alien biological invasion, weeds and pest damage is increasing. A variety of adversity stresses exist in the growing season of crops at the same time, causing serious losses to crop production

Method used

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  • Compound, preparation method and application thereof and product containing compound
  • Compound, preparation method and application thereof and product containing compound
  • Compound, preparation method and application thereof and product containing compound

Examples

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preparation example Construction

[0047] In addition, the present invention also provides a preparation method of the above compound, including:

[0048] Fermenting Chaetomium globosa or spores of Chaetomium globosa to obtain a fermentation product containing the compound, and separating and purifying the fermentation product to obtain the compound.

[0049] Among them, the microbiological characteristics of Chaetomium globosa are: after being cultured on a PDA plate at 28° C. for 7 days, the colonies appear white, and most of them are vegetative hyphae. Its vegetative body is colorless or light brown mycelium, and there is a diaphragm between each cell of the mycelium. After 14 days, it can be seen that simple long and erect conidiophores can be seen on the aerial mycelia, and the colony is dark brown at the same time, and agglomerated mycelia can be seen under the microscope.

[0050] The invention also provides the application of the Chaetomium globosa in regulating the stress resistance of plants.

[005...

Embodiment 1

[0065] The isolation and identification of embodiment 1 chaetomium globosa bacterial strain

[0066] Eleven strains of fungi were isolated from the soil of Weijia Farm in Nong'an County, Jilin Province by applying PDA+penicillin and streptomycin plates, one of which was the Chaetomium globosa, which can produce light yellow on the PDA agarose plate compound. The strain was identified as Chaetomium globosum by taxonomic research and molecular biology research.

[0067] The microbiological characteristics of the bacterium are as follows: after being cultured on a PDA plate at 28° C. for 7 days, the colonies appear white, and most of them are vegetative hyphae. Its vegetative body is colorless or light brown mycelium, and there is a diaphragm between each cell of the mycelium. After 14 days, it can be seen that simple long and erect conidiophores can be seen on the aerial mycelia, and the colony is dark brown at the same time, and agglomerated mycelia can be seen under the micr...

Embodiment 2

[0076] Example 2 Isolation and identification of azaronin A in the fermentation product of Chaetomium globosa

[0077] (1) Preparation and separation method of Chaetomium globosa fermentation product

[0078] Preparation of ferments:

[0079] Get the spores of Chaetomium globus and list them on the agar plate (g / L) of 8L PDA: get yeast extract 0.5g, glucose 10.0g, calcium phosphate 5.0g, ammonium sulfate 0.5g, potassium chloride 0.2g, Magnesium sulfate 0.1g, manganese sulfate 0.0001g, ferrous sulfate 0.0001g, agar 15.0g, the plate was incubated at room temperature (20-22°C) for 14 days. When the spores were observed to turn black, they were extracted with methanol to obtain a Chaetomium globosa fermentation product.

[0080] Reverse-phase liquid chromatography separation and purification:

[0081] The above 8L fermentation product solution was evaporated to a volume of 2L under reduced pressure, and then extracted three times with equal amounts of ethyl acetate, the extract...

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Abstract

The invention provides a compound, a preparation method and an application thereof and a product containing the compound and relates to the technical field of compounds. The novel compound is of a structure shown as formula (I), and experiments prove that the compound can effectively regulate stress resistance of plants and improve adversity stress resistant capacity of the plants. Therefore, the product containing the compound can also have the function of regulating stress resistance of the plants. The invention further provides the preparation method of the compound. The compound is prepared from fermentation products of chaetomium globosum or chaetomium globosum spores through separation and purification. The operation is simple, the practicability is high, the blank of shortage of related research in regulation of stress resistance of the plants by metabolites in soil microorganisms is filled up, and one novel method and thought are provided for utilization of the soil microorganisms and particularly improvement of the stress resistant capacity of the plants in future.

Description

technical field [0001] The present invention relates to the technical field of compounds, in particular to a compound, a preparation method of the compound, an application of the compound and a product using the compound. Background technique [0002] Soil microorganisms spend all or part of their life course in the soil, and play an important role in various processes in the soil, which affect human social and economic development in many ways. With the change of global climate, high temperature, low temperature, drought and waterlogging will occur alternately and frequently, and the environmental pressure caused by soil salinization, heavy metal pollution, alien biological invasion, weeds and pest damage is increasing. A variety of adversity stresses exist simultaneously in the growing season of crops, causing serious losses to crop production. [0003] Many soil microorganisms can secrete secondary metabolites, such as rhizobia secreting rhizobia nodulation factor - lipo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D217/04A01P21/00A01N43/42C12P17/12C12R1/645
CPCA01N43/42C07D217/04C12P17/12
Inventor 康耀卫李想
Owner 内蒙古康元生物技术有限公司
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