Method for quickly analyzing main flavonoid aglycones of seabuckthorn through RP-HPLC-DAD

Abstract

The invention relates to the technical field of analytical chemistry and discloses a method for quickly determining the content of catechinic acid, rutin, myricetin, quercetin, kaempferol and isorhamnetin in seabuckthorn by utilizing reversed high performance liquid chromatography diode array detection. The method comprises the following steps: diluting a main component standard substance or extract of seabuckthorn flavone by using methyl alcohol, by using octadecyl silane bonded silica gel filler as a stationary phase, and using methyl alcohol-water (acidic) as a mobile phase, performing isocratic elution, and then recording a chromatogram under all the flavone absorption wavelengths. According to the method, the octadecyl silane bonded silica gel filler is used as the stationary phase and methyl alcohol-water (acidic) is used as the mobile phase, so that almost all the flavonoid aglycones components in the seabuckthorn flavone extract can be quantitatively determined, the base line separation for all the components almost can be realized and the accurate quality control on the seabuckthorn flavone extract can be effectively realized. According to the method provided by the invention, the specificity is high, the sensitivity is high and the accuracy is high.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine production quality detection, in particular to a content determination method of six main flavonoid aglycones in seabuckthorn and its application in extraction and analysis. Background technique [0002] Seabuckthorn is a shrub or small tree belonging to the genus Sourthorn in the family Echinacea, also known as vinegar willow, black thorn, sour thorn, Qicharigana (Mongolian name), Dapu (Tibetan name), and Jihan (Uyghur name). Seabuckthorn is mainly distributed in Asia and Europe, and its various tissues, especially the berries, are used in traditional medicines in Tibet, China, Mongolia, and the Middle East. In 1977, seabuckthorn was included in the Pharmacopoeia of the People's Republic of China, and later included in the Chinese Pharmacopoeia. [0003] Flavonoids are one of the main active ingredients of seabuckthorn. Seabuckthorn flavonoids have been proven to have antiox...

AI Technical Summary

Problems solved by technology

At present, the extraction and analysis methods of seabuckthorn flavonoids are mainly based on the simple analysis of total flavonoids, but there is a lack of quantitative analysis of classified flavonoid aglycones in the corresponding extracts, and it is impossible to achieve accurate quality control and in-depth pharmacological efficacy analysis of the extracted components.

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