Kit for detecting resistance to pituitary adenoma radiotherapy through protein marker

A protein marker, pituitary adenoma technology, applied in the field of kits for detecting pituitary adenoma radiotherapy resistance

Inactive Publication Date: 2017-12-05
BEIJING NEUROSURGICAL INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no domestic and foreign literature reports on the molecular markers for identifying and predicting radiotherapy sensitivity of pituitary adenomas. Therefore, in clinical treatment, the discovery of such markers is urgently needed to better serve the radiotherapy of pituitary adenomas.

Method used

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  • Kit for detecting resistance to pituitary adenoma radiotherapy through protein marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Specimen preparation:

[0030] (1) Pituitary tumor specimens were taken (pituitary tumor specimens were obtained from biopsy or surgery, and the specimens received radiation therapy before the experiment. The central dose of Gamma Knife was 26-33Gy, the peripheral dose was 13-16Gy, and the time was 50-60 minutes. ) for wax block embedding: 70%, 80%, 90%, 95%, and 95% ethanol were placed for 1 hour each; 100% ethanol was placed for 30 minutes, and repeated; xylene was placed for 20 minutes, and repeated; immersed in wax liquid 30 minutes, repeat twice.

[0031] (2) Section the specimen wax block with a thickness of about 5 μm.

[0032] (3) Place the slices in an oven at 56-60 degrees for about 0.5-1 hour.

[0033] Immunohistochemistry:

[0034] (1) Use the Leica automatic immunohistochemical instrument (model LEICA BONDⅢ) to label the detected protein on the computer, and at the same time operate the working conditions of the detected protein antibody and the antibody...

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Abstract

The invention provides the use of a reagent for detecting the expression level of a protein marker in preparing a product for detecting radiotherapy resistance of a pituitary adenoma, as well as a kit and protein marker combination for detecting the radiotherapy resistance of a pituitary adenoma. The present invention can detect the sensitivity of the treated object to radiotherapy of pituitary adenoma by detecting the expression level of protein markers, and analyze whether there is radiotherapy resistance, thereby providing an effective basis for predicting the effect of patients receiving radiotherapy and guiding clinical diagnosis and treatment.

Description

technical field [0001] The invention relates to the field of pituitary adenoma protein markers, in particular to the use of protein markers in pituitary adenoma radiotherapy resistance and a kit for detecting pituitary adenoma radiotherapy resistance through protein markers. Background technique [0002] Pituitary adenomas are common tumors of the nervous system, accounting for about 10%-25% of intracranial tumors. Pituitary adenomas can be divided into functioning adenomas and nonfunctioning adenomas. Functional pituitary tumors are also called secretory pituitary tumors, which are divided into prolactinomas, growth hormone adenomas, corticotropic hormone adenomas, thyrotropin adenomas, etc. These adenomas manifest as excessive secretion of pituitary hormones, such as growth hormone , prolactin, etc. Partially functional pituitary tumors have aggressive biological characteristics, and are prone to residual and recurrence after surgery. Non-functioning pituitary adenoma i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68G01N33/6893
Inventor 李阳芳张亚卓李储忠刘阿力
Owner BEIJING NEUROSURGICAL INST
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