Method for high-efficiency multiplication of test-tube tomato seedlings

Abstract

The invention provides a method for high-efficiency multiplication of test-tube tomato seedlings and belongs to the technical field of plant tissue culture. The method comprises the following steps of: treatment of a mother bottle of the test-tube tomato seedlings, addition of a liquid medium, germination and culture of axillary buds and culture of new test-tube seedlings. The method provided by the invention has the beneficial effects that the germination of the axillary buds of the obtained test-tube tomato seedlings can be obviously accelerated, the seedling formation time of the tomato bud seedlings can be shortened, a large amount of test-tube tomato seedlings can be rapidly obtained in a shorter time, the obtained test-tube tomato seedlings have the advantages of strong growing power and high quality, the subculturing times of the test-tube tomato seedlings can be reduced, and the increase of the mutation ratio of the test-tube tomato seedlings can be delayed, so that technical support is provided for large-scale production of the tomato seedlings by utilizing a plant-tissue culture technology.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a method for efficient proliferation of tomato test-tube seedlings. Background technique [0002] Tomato (Lycopersicum esculentum Mill) is an annual herbaceous plant belonging to the genus Tomato of the Solanaceae family, and is the main cultivated vegetable or vegetable fruit. In terms of production, tomato seeds are almost F1 hybrids, which need to be produced every year or imported from abroad. Using tissue culture technology to rapidly propagate tomato seedlings can reduce production costs, maintain their excellent characters, and provide a large number of seedlings to the market in a short period of time, which has high economic value. However, the apical dominance of test-tube seedlings of tomato is very strong, and it rarely produces cluster buds or buds from nodes during proliferation. Usually, stems are used for propagation, so that the reproduction coeffic...

AI Technical Summary

Problems solved by technology

However, the top advantage of tomato test-tube plantlets is very strong. When multiplying, clustered buds are rarely produced or buds grow from nodes. Usually, stem segments are used for propagation, resulting in a low reproductive coefficient, which cannot achieve the purpose of rapid propagation in production.

In tomato test-tube seedling multiplication and cultivation, the whole plant of sterile seedlings is usually taken out, the sprouts of the upper stem are cut, and the base is discarded, which causes material waste to a certain extent.

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