FB1(fumonisin B1) matrix standard substance and preparation method thereof

A standard substance and matrix technology, which is applied in the field of fumonisin B1 matrix standard substance and its preparation, can solve problems such as the preparation method of FB1 matrix standard substance, and achieve the effect of promoting international trade, ensuring accuracy, and breaking trade barriers

Inactive Publication Date: 2017-12-15
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no FB 1 Report on matrix reference material and its preparation method

Method used

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  • FB1(fumonisin B1) matrix standard substance and preparation method thereof
  • FB1(fumonisin B1) matrix standard substance and preparation method thereof
  • FB1(fumonisin B1) matrix standard substance and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Materials, instruments and reagents

[0029] 1.1 Materials

[0030] Corn flour (tested by the method of 2.6, it does not contain FB 1 ) was purchased from a large supermarket in Shanghai, and Fusarium moniliforme was provided by the Institute of Agricultural Product Quality Standards and Testing Technology, Shanghai Academy of Agricultural Sciences.

[0031] Facebook 1 Standards (50 μg / mL) were purchased from Sigma-Aldrich (St.Louis, MO, USA).

[0032] 1.2 Medium

[0033] Potato Dextrose Agar Medium (PDA): Weigh 40.0g of PDA powder into 1L of distilled water, heat and boil until completely dissolved, and autoclave at 121°C for 20min for later use.

[0034] Corn medium: Weigh 50g of corn flour into a 250mL Erlenmeyer flask, add 20mL of distilled water and mix well, seal the mouth of the bottle with air-permeable sealing film, soak overnight, autoclave (121°C, 20min), shake the corn flour after cooling and set aside .

[0035] 1.3 Main instruments

[0036] Water...

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Abstract

The invention provides a preparation method of an FB1(fumonisin B1) matrix standard substance. The method comprises steps as follows: toxigenic fungus strains of FB1 are inoculated to a culture medium of edible agricultural products and cultured for toxin production, and the highly toxigenic culture medium is lyophilized by a lyophilizer, smashed and screened; blank edible agricultural product powder is added according to the principle of geometric-proportion dilution, FB1 is enabled to reach the quantity level, the materials are mixed uniformly, and the edible agricultural product matrix standard substance of FB1 is obtained. According to the prepared edible agricultural product matrix standard substance of FB1, a value traceability substance is provided for daily FB1 residue detection, the problem of inaccurate detection results caused by matrix difference is solved, and monitoring for FB1 in the agricultural products such as corn, wheat, rice and the like is accurately guaranteed.

Description

technical field [0001] The invention relates to the technical field of agricultural product safety detection, in particular to a fumonisin B 1 Matrix standard substance and its preparation method. Background technique [0002] Fumonisins are mainly water-soluble secondary metabolites produced by Fusarium moniliforme under certain temperature and humidity conditions. So far, eleven types of fumonisins have occurred, the most important of which is fumonisin B. 1 (FB 1 ). Research shows that intake of FB 1 Can cause equine leukoencephalomalacia (EquineLeucoencephalomalacia, ELEM), porcine pulmonary edema syndrome (Porcine Pulmonary Edema, PPE), toxicity studies on mice show that FB 1 May cause damage to liver or kidneys. In addition, FB 1 It also has strong cytotoxicity and immunotoxicity, and is closely related to the occurrence of human esophageal cancer. Therefore, the International Agency for Research on Cancer (IARC) put FB 1 Listed as a Class 2B carcinogen as a po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N1/38
CPCG01N1/28G01N1/286G01N1/38G01N2001/2893
Inventor 韩铮赵志辉郭文博聂冬霞何卫警杨俊花
Owner SHANGHAI ACAD OF AGRI SCI
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