A kind of preparation method of Hong Kong oyster tetraploid larvae

Abstract

The invention discloses a preparation method of young mollusks of Hongkong tetraploid oysters. The method comprises the following technical steps of selectively choosing triploid eggs, carefully choosing diploid sperms, inhibiting second polar bodies of oosperms, obtaining tetraploid young mollusks, and raising tetraploid progenies. The feature of normal reduction mitosis of some triploid eggs is adopted so as to create a raising method for preparing tetraploid oysters by inhibiting second polar bodies of oosperms (triploid eggs +diploid sperms). The method differs from a method for obtaining tetraploid oysters by inhibiting first polar bodies of oosperms. The preparation method makes a great difference in a traditional preparation mode and helps break the monopolies of the US, France, Korea and other foreign countries on raisins technology of tetraploid oysters. It is the first time to obtain livable young mollusks of Hongkong tetraploid oysters. The method makes the biological method of producing 100% Hongkong triploid oysters feasible, promote the steady development of the industry of Hongkong oysters in the coastal region of southern China and offers the possibility of achieving improved varieties of the industry. The preparation method of young mollusks of Hongkong tetraploid oysters is being advantaged by being implementable, reusable, and applicable.

Description

Technical field: [0001] The invention belongs to the technical field of shellfish genetics and breeding in marine agriculture, and in particular relates to a preparation method of Hong Kong oyster tetraploid juvenile shellfish. Background technique: [0002] A tetraploid is a cell or organism that has four sets of chromosomes, as opposed to a diploid organism that contains only two sets of chromosomes, or a triploid organism that contains three sets of chromosomes. In nature, natural tetraploid mollusks have hardly been reported, and artificially induced tetraploids are mainly concentrated in oysters, scallops, mussels and other species. Usually, the tetraploid production method includes inhibiting the release of the polar body of the fertilized egg, inhibiting the first cleavage, promoting the chromosome doubling of the normal ploidy fertilized egg through cell fusion or gynogenesis, and forming a tetraploid through embryonic development. After years of research, scholars ...

AI Technical Summary

Problems solved by technology

After years of research, scholars have found that by using triploid eggs to fertilize diploid sperm and inhibiting the release of the first polar body of fertilized eggs, tetraploid adults of surviving shellfish can be obtained. Other methods are currently not applicable, although most Both methods can obtain tetraploid larvae, but the larvae cannot metamorphose into tetraploid juveniles, resulting in the inability to carry out follow-up research

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