Rapid scanning and imaging method of three-dimensional motion platform for cell DNA ploidy analysis

A three-dimensional movement and fast scanning technology, which is applied in the analysis of materials, material analysis through optical means, instruments, etc., can solve the problems of low work efficiency and long acquisition time, and achieve the effect of improving efficiency and shortening scanning time

Active Publication Date: 2020-03-06
山东颐泽天泰医疗科技有限公司
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  • Abstract
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Problems solved by technology

Long collection time and low work efficiency

Method used

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  • Rapid scanning and imaging method of three-dimensional motion platform for cell DNA ploidy analysis
  • Rapid scanning and imaging method of three-dimensional motion platform for cell DNA ploidy analysis
  • Rapid scanning and imaging method of three-dimensional motion platform for cell DNA ploidy analysis

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Embodiment Construction

[0026] like figure 1 The shown three-dimensional motion platform for cell DNA ploidy analysis includes an image information acquisition system 1, a microscope motion control system 2 and a computer 3, and the computer 3 is equipped with an image analysis system and a MySQL database. Among them, the image acquisition system 1 is used for the acquisition of the position calibration information of the whiteboard slides in the early stage, and the scanning and imaging of the DNA of the cells during the formal image scanning in the later stage, and the imaged images are stored in the MySQL database in the computer 3 . The microscope motion control system 2 realizes the motion control of the X-axis, Y-axis, and Z-axis of the microscope through the motion control software of the microscope loading platform, and can quickly locate the calibration position of the whiteboard slide, and can quickly locate the maximum gradient value and The location of the minimum gradient value. The ima...

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Abstract

The invention discloses a rapid scanning imaging method of a 3D motion platform for cell DNA ploidy analysis. An image on a glass slide is rapidly scanned, the average variable quantity of gradient value of each step motion of the Z axis is calculated according to gradient value data during each change of the Z axis, the difference between the gradient value at the moment and the maximum gradientvalue is calculated through calculation of gradient values of all scanned images, the number of steps between positions of the Z axis when the position distance of the Z axis at the moment is the maximum gradient value is calculated, and the number of the steps is taken as a compensation basis. The average change quantity of the gradient value is multiplied with the number of the steps, the gradient value required to be compensated is obtained for image compensation, and the technical effect identical to focusing is obtained. Compared with the prior art, the rapid scanning imaging method of the 3D motion platform for cell DNA ploidy analysis is applicable to the processing process of batch scanning of images, the whole scanning process of one glass slide only requires one focusing, the scanning time is shortened, and the efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of three-dimensional motion platform manipulation methods, and relates to a fast scanning imaging method for a three-dimensional motion platform for cell DNA ploidy analysis. Background technique [0002] When the microscope scans the image, it needs to adjust the focal length to find the clearest image. The focusing process is time-consuming and laborious. In order to focus quickly and improve precision, existing microscopes often use the following methods to improve efficiency. The first way is "metallographic three-dimensional automatic platform" (Zeng Libo et al.: "Metallographic three-dimensional automatic platform", "Metal Heat Treatment", Volume 27, No. 9, 2002.) The article introduces the control of the loading platform through software. The movement in the X-axis, Y-axis, and Z-axis directions, automatic movement, and automatic focus have changed the algorithm of the automatic focus, and the algori...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84G01N21/01
Inventor 李庆华夏啟凯刘霞
Owner 山东颐泽天泰医疗科技有限公司
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