Method for extracting general flavone from herba lysimachiachristinae hance

A technology of money grass and total flavonoids, which is applied in the direction of medical formula, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problems of increased cost, low selectivity, complicated process, etc.

Inactive Publication Date: 2018-02-09
桂林纽泰生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the common solvent C0 2 It has the characteristics of non-polarity and small relative molecular mass, which makes the extraction effect unsatisfactory due to its low solubility and low selectivity for some substances. In actual operation, it is often necessary to increase the extraction rate by adding entraining agents. Finally, it is necessary to try to remove the entraining agent in the volatile oil, which will inevitably lead to the complexity of the process and the loss and oxidation of volatile components; in addition, because water is insoluble in CO 2 , so the supercritical C0 2 The extraction process often needs to pre-dry the raw materials, which increases the cost and causes the loss of aromatic compound oil
In addition, when extracting volatile oils from plants, due to supercritical CO 2 It has similar solubility not only to volatile oils but also to low-polarity compounds such as waxes, fatty acids, colored substances and resins in the stratum corneum. These substances will also be extracted during the extraction process, although complex systems can also be used. Pure volatile oil, but the operation is cumbersome and the requirements for equipment are more stringent

Method used

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  • Method for extracting general flavone from herba lysimachiachristinae hance
  • Method for extracting general flavone from herba lysimachiachristinae hance
  • Method for extracting general flavone from herba lysimachiachristinae hance

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Effect test

Embodiment 1

[0023] The method for extracting total flavonoids from Desmodium, specifically comprises the following steps:

[0024] 1) Clean the money grass, dry it, pulverize it with a pulverizer, and pass through a 20-mesh sieve;

[0025] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 150-250°C, pressure 8-10Mpa, extraction time 0.5h, extract Desmodium sativa, after the extraction is completed, release the pressure, the extract flows into the collection tank and cools to room temperature to obtain the extract;

[0026] 3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through DM130 macroporous resin, a...

Embodiment 2

[0028] The method for extracting total flavonoids from Desmodium, specifically comprises the following steps:

[0029] 1) Clean the money grass, dry it, pulverize it with a pulverizer, and pass through a 40-mesh sieve;

[0030] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 150-250°C, pressure 8-10Mpa, extraction time 1.0h, extract Desmodium sativa, after the extraction is completed, release the pressure, the extract flows into the collection tank and cools to room temperature to obtain the extract;

[0031] 3) Purification: Concentrate the extract obtained in step 2), control the temperature to ≤50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, and perform column chromatography through AB-8 macroporous res...

Embodiment 3

[0033] The method for extracting total flavonoids from Desmodium, specifically comprises the following steps:

[0034] 1) Clean the money grass, dry it, pulverize it with a pulverizer, and pass through an 80-mesh sieve;

[0035] 2) Subcritical water extraction: place the processed raw materials in an extraction kettle, add hydroxyethyl cellulose equivalent to 0.001 parts by weight of the raw materials, inject deoxygenated deionized water into the kettle at a flow rate of less than 10mL / min, and extract at a temperature of 150-250°C, pressure 8-10Mpa, extraction time 1.0h, extract Desmodium sativa, after the extraction is completed, release the pressure, the extract flows into the collection tank and cools to room temperature to obtain the extract;

[0036]3) Purification: Concentrate the extract obtained in step 2), control the temperature ≤ 50°C, control the vacuum at -0.08±0.01MPa, concentrate to 0.95-1.05g / ml, perform column chromatography through D101 macroporous resin, an...

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Abstract

The invention discloses a method for extracting general flavone from herba lysimachiachristinae hance. The method comprises the following steps of (1) cleanly cleaning the herba lysimachiachristinae hance; drying the herba lysimachiachristinae hance; pulverizing the herba lysimachiachristinae hance by a pulverizer; performing sieving; (2) performing subcritical water extraction: putting the treated raw materials into an extraction kettle; adding hydroxyethyl cellulose accounting for 0.001 weight part of the raw materials; injecting deoxygenated deionized water into the kettle; extracting the herba lysimachiachristinae hance under the conditions that the extraction temperature is 150 to 250 DEG C, the pressure is 8 to 10Mpa and the extraction time is 0.5 to 1.0h; after the extraction is finished, performing pressure relief; enabling extraction liquid to flow into a collecting tank; performing cooling to room temperature to obtain extraction liquid; (3) performing purification: concentrating the extraction liquid obtained in the second step; performing column chromatography through macroporous resin; performing elution by 50-percent v/v ethyl alcohol to obtain elution liquid; performing concentration and drying to obtain a finished product.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting total flavonoids from Desmodium. Background technique [0002] Lysimachia christinae Hance is the fresh or dry whole plant of Primulaceae plant Lysimachia christinae Hance, mainly produced in Jiangsu, Guangdong, Sichuan and Guangxi. In addition, it is also produced in Zhejiang, Hunan, Fujian and other places. Desmodium contains phenolic components, flavonoids, glycosides, tannins, volatile oils, amino acids, choline, sterols, potassium chloride, lactones and other components. According to literature reports, Desmodium moss has the effect of removing stones, and is mainly used to treat damp-heat jaundice, liver and gallstones, urinary tract stones, bacterial infections and other diseases. [0003] The extraction of flavonoids in the prior art mainly adopts the ultrasonic method to extract flavonoids. Its principle is that the cavitatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K47/38
CPCA61K36/185A61K47/38A61K2236/37A61K2236/51A61K2236/55
Inventor 谢冬养
Owner 桂林纽泰生物科技有限公司
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