Method for regulating diapause of lysiphlebus testaceipes

A technology of diapause and diapause of aphids, which is applied in the field of regulating and controlling the diapause of Aphids aphids, can solve the problems of long-term storage of bee sources, inability to release in time, high reproduction costs, etc., and achieve the effect of improving the level of scientific management

Inactive Publication Date: 2018-02-16
GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Each generation lasts about 1 month, and the low-temperature storage period of aphids is generally within 1 month. If it exceeds one month, the emergence rate of aphids and the amount of eggs laid by adult bees will be greatly reduced. Therefore, special personnel must insist on subculture all year round, and The increase of breeding algebra will lead to the degeneration of bee species, unable to continue to multiply, and the cost of reproduction will be high
In actual production and application, the bee source cannot be stored for a long time and cannot be released in a timely manner, which has become a technical bottleneck restricting the large-scale propagation and application of T.

Method used

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  • Method for regulating diapause of lysiphlebus testaceipes
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  • Method for regulating diapause of lysiphlebus testaceipes

Examples

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Effect test

Embodiment 1

[0037] Embodiment 1, the induction of diapause of Aphidia japonica

[0038] 1. Determination of diapause-induced sensitive insect state of T. chinensis

[0039] 1. Dealing with worm state

[0040] T. teapodoides is a parasitic natural enemy insect with complete metamorphosis, and has four stages of development: egg, larva, pupa and adult. Due to the difficulty in age classification, the first eclosion of T. chinensis was placed under the conditions of 25±1°C and relative humidity of 80±10%. After 24 hours, the host alfalfa larvae were parasitized, and the parasitic eggs continued to be cultivated for 72, 120 and 168 hours, which is equivalent to dividing the insect stages into eggs, young larvae (1-2 instars), advanced larvae (3-4 instars) and pupae according to the time of culture and development, and randomly dissected 50 parasitic alfalfa aphids , combined with the morphological characteristics of the parasitized alfalfa aphid as the standard for judging the developmental a...

Embodiment 2

[0060] Embodiment 2, the effect of low temperature storage treatment on the diapause relief of T.

[0061] By measuring the eclosion rate of the diapausing aphid of T. teasporum, as well as the lifespan and parasitism rate of adult bees after eclosion, the time of low-temperature storage for the diapause of T. tea was determined. The specific steps are as follows: the diapause aphid (pupa) is collected in a petri dish (diameter 6cm, height 2cm), and the top is covered with an air-permeable gauze. Diapause individuals were induced for 30 days under the conditions of 8℃, photoperiod L8:D16, relative humidity 70-80%, and light intensity 4000-5000Lx. In the test, four refrigeration periods of 30, 60, 90 and 120 days were set up, and they were stored in a refrigerator at 4°C, in the dark, and with a relative humidity of 70-80%. Take out 100 diapausing aphids from the refrigerator every 30 days, put them into an artificial climate box with a temperature of 25°C, a photoperiod of L1...

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Abstract

The invention discloses a method for regulating diapause of lysiphlebus testaceipes. The method comprises the steps of diapause induction and diapause storage, wherein in the step of diapause induction, larvae of high instar (3-4 instars) are induced under the conditions that the temperature is 8-10 DEG C, and the light period is L8:D16, and after the hosts, namely aphis medicagini, become mummy aphids, lysiphlebus testaceipes diapause pupas are obtained, wherein the diapause rate of the lysiphlebus testaceipes induced by using the method can reach 70% or above; in the step of diapause storage, the obtained lysiphlebus testaceipes mummies entering a diapause state are transferred into the dark environment where the temperature is 4 DEG C and stored for 90 days, and the eclosion rate and the service life of adult lysiphlebus testaceipes has no significant difference with non-diapause mummies in parasitism. By means of the method, the preservation period of the lysiphlebus testaceipes can be effectively prolonged by three times or above, and the regulation of the development progress of the lysiphlebus testaceipes is achieved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for regulating the diapause of Aphidus chinensis. Background technique [0002] Alfalfa aphid is an explosive agricultural pest with worldwide distribution. This insect can harm more than 400 kinds of plants, and its generations overlap seriously. The harm of alfalfa aphid is increasing year by year, coupled with the frequent use of chemical pesticides, the drug resistance of alfalfa aphid is increasing year by year, and it cannot be effectively controlled. Therefore, it is urgent to improve the control technology of alfalfa aphid in production and application. The use of parasitic wasps and other natural enemies of pests to control pests is an important biological control method of pests. The wasp wasp is an important parasitic natural enemy of the alfalfa aphid. The wasp lays eggs in the body of the alfalfa aphid. After the eggs hatch, the larvae of the wasp ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00A01K67/033
Inventor 刘爱萍孙程鹏李钢铁任卫波高书晶徐林波张爱萍
Owner GRASSLAND RES INST OF CHINESE ACAD OF AGRI SCI
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