Method for producing in-vitro embryos by aid of superovulation mother cells of cows

A technology of superovulation and mother cells, which is applied in veterinary instruments, medical science, animal delivery and other directions, can solve the problems of low efficiency and low success rate in the operation of external embryos of cows, improve production efficiency and economic benefits, and the method is simple , the effect of easy operation

Inactive Publication Date: 2018-02-23
新疆豪子畜牧有限公司
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Problems solved by technology

[0004] The present invention proposes a method for producing in vitro embryos by using cow superovulated oocytes, which solves the low efficiency of cow ectoembryo operations and the For the

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  • Method for producing in-vitro embryos by aid of superovulation mother cells of cows

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Embodiment 1

[0018] Such as figure 1 As shown, the present invention provides a method for producing in vitro embryos using cow superovulated oocytes, including drug-induced multiple follicle development process 1, vaginal ultrasound egg retrieval process 2, in vitro fertilization and embryo culture process 3, embryo transfer process 4 and Luteal phase support process 5 drug-induced multiple follicle development process 1 is to scan the cow’s vagina with ultrasound and confirm the ovarian status, and administer medication when appropriate, and recheck the cow’s vaginal ultrasound after a week of medication. If follicle activation is found, cyst puncture should be performed. Inject gonadotropin or follicle-stimulating hormone to stimulate the development of multiple follicles every week. During ovulation induction, use vaginal ultrasound and blood sex hormone measurement to monitor follicle development. Left and right transvaginal ultrasound for egg retrieval. Gonadotropins are generally d...

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Abstract

The invention discloses a method for producing in vitro embryos by using superovulated oocytes of cows, which sequentially carries out the process of drug-induced multi-follicle development, the process of egg retrieval by vaginal ultrasound, the process of in vitro fertilization and embryo culture, the process of embryo transplantation and the process of corpus luteum support and precise Superovulation of cows and production of in vitro embryos are carried out with advanced operation and strict time control, and local anesthesia is given to cows in the vaginal ultrasound egg retrieval process and embryo transfer process, so that they can cooperate with egg retrieval and fertilized egg implantation, luteal phase support The process ensures that the fertilized eggs in the cow can safely transform into calves, thereby improving the breeding efficiency of cows to calves, and solving the problem that people currently produce in vitro embryos from cow superovulated mother cells based on their own experience and feelings. The resulting problems of low efficiency and low success rate of ectoembryo manipulation, as well as improving the economic benefits of livestock producers, have very good practical value.

Description

technical field [0001] The invention relates to a method for producing in vitro embryos, in particular to a method for producing in vitro embryos by using cow superovulated oocytes. Background technique [0002] Cattle are animals that provide a very powerful boost to human production and development. At first, people tamed bison and raised them for meat to increase nutrition. Later, people discovered that cattle can cultivate land, so they became farmers' helpers and promoted economic development and social progress. In modern times, people have discovered that cow's milk is a source of high-quality, low-cost and stable supply of dairy products. Therefore, raising beef cattle and dairy cattle has become a very important industry. However, compared with other livestock, cattle have limited fertility, which restricts the rapid development of animal husbandry. Therefore, people adopt superovulation technology to promote the ovulation quantity of cattle. Studies have shown t...

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Application Information

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IPC IPC(8): A61D19/04
CPCA61D19/04
Inventor 孙岩马雄亚贾旭升牛志宏王旭东周程
Owner 新疆豪子畜牧有限公司
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