Method for producing in-vitro embryos by aid of superovulation mother cells of cows

Abstract

The invention discloses a method for producing in vitro embryos by using superovulated oocytes of cows, which sequentially carries out the process of drug-induced multi-follicle development, the process of egg retrieval by vaginal ultrasound, the process of in vitro fertilization and embryo culture, the process of embryo transplantation and the process of corpus luteum support and precise Superovulation of cows and production of in vitro embryos are carried out with advanced operation and strict time control, and local anesthesia is given to cows in the vaginal ultrasound egg retrieval process and embryo transfer process, so that they can cooperate with egg retrieval and fertilized egg implantation, luteal phase support The process ensures that the fertilized eggs in the cow can safely transform into calves, thereby improving the breeding efficiency of cows to calves, and solving the problem that people currently produce in vitro embryos from cow superovulated mother cells based on their own experience and feelings. The resulting problems of low efficiency and low success rate of ectoembryo manipulation, as well as improving the economic benefits of livestock producers, have very good practical value.

Description

technical field [0001] The invention relates to a method for producing in vitro embryos, in particular to a method for producing in vitro embryos by using cow superovulated oocytes. Background technique [0002] Cattle are animals that provide a very powerful boost to human production and development. At first, people tamed bison and raised them for meat to increase nutrition. Later, people discovered that cattle can cultivate land, so they became farmers' helpers and promoted economic development and social progress. In modern times, people have discovered that cow's milk is a source of high-quality, low-cost and stable supply of dairy products. Therefore, raising beef cattle and dairy cattle has become a very important industry. However, compared with other livestock, cattle have limited fertility, which restricts the rapid development of animal husbandry. Therefore, people adopt superovulation technology to promote the ovulation quantity of cattle. Studies have shown t...

AI Technical Summary

Problems solved by technology

[0004] The present invention proposes a method for producing in vitro embryos by using cow superovulated oocytes, which solves the low efficiency of cow ectoembryo operations and the For the problem of low success rate, precise operation and strict time control are used to superovulate cows and produce in vitro embryos, thereby improving production efficiency and economic benefits

Images