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Preparation method and applications of Eleutherine plicata Herb effective parts for treating coronary heart disease

A technology of effective parts and small red garlic is applied in the field of preparation of effective parts of small red garlic, and can solve the problems of no preparation method of effective parts of small red garlic and the like

Active Publication Date: 2018-02-23
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no complete and effective preparation method for the effective part of red garlic in the current technology, and there is no process research report on its purification using macroporous adsorption resin

Method used

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  • Preparation method and applications of Eleutherine plicata Herb effective parts for treating coronary heart disease
  • Preparation method and applications of Eleutherine plicata Herb effective parts for treating coronary heart disease
  • Preparation method and applications of Eleutherine plicata Herb effective parts for treating coronary heart disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Take small red garlic, add 8 times the amount of 80% ethanol, soak for 30 minutes, reflux twice, each time for 2 hours, filter, combine the extracts, concentrate under reduced pressure to 0.1g crude drug / mL, adjust the pH to 3, and use it as the sample solution;

[0087] The loading liquid is dynamically adsorbed by the macroporous adsorption resin D-101, the loading speed is 2BV / h, and the resin diameter-to-height ratio is 1:8. After the adsorption is completed, wash with water until neutral, discard the washing liquid, and then add 8BV 80% ethanol is used for dynamic analysis, and the analysis speed is 1BV / h. The analysis liquid is concentrated under reduced pressure to recover the solvent, and the effective parts of the small red garlic are obtained after vacuum drying. Among them, the total naphthoquinone content in the effective part is measured by UV direct method, which is 50%; isofulvedin, shallotine and shallot A are determined by high performance liquid chromat...

Embodiment 2

[0089] Take small red garlic, add 10 times the amount of 85% ethanol, soak for 40 minutes, reflux twice, each time for 2 hours, filter, combine the extracts, concentrate under reduced pressure to 0.2g crude drug / mL, adjust the pH to 4, and use it as the sample solution;

[0090] The loading liquid is dynamically adsorbed through the macroporous adsorption resin X-5, the loading speed is 2BV / h, and the diameter-to-height ratio of the resin is 1:6. After the adsorption is completed, wash with water until neutral, discard the washing liquid, and then add 10BV 85% ethanol is used for dynamic analysis, the analysis speed is 2BV / h, the analysis solution is concentrated under reduced pressure to recover the solvent, and the effective part is obtained after vacuum drying. Among them, the total naphthoquinone content in the effective part is measured by UV direct method, which is 52%; isofulvedin, shallot and shallot A are determined by high performance liquid chromatography, and the co...

Embodiment 3

[0092] Take small red garlic, add 10 times the amount of 85% ethanol, soak for 30 minutes, reflux twice, each time for 2 hours, filter, combine the extracts, concentrate under reduced pressure to 0.2g crude drug / mL, adjust the pH to 4, and use it as the sample solution;

[0093] The loading liquid is dynamically adsorbed by the macroporous adsorption resin D-101, the loading speed is 1BV / h, and the diameter-to-height ratio of the resin is 1:8. After the adsorption is completed, wash with water until neutral, discard the washing liquid, and then add 10BV Dynamic analysis was performed with 90% ethanol at a rate of 3BV / h. The analysis solution was concentrated under reduced pressure to recover the solvent, and the effective fraction was obtained after vacuum drying. Among them, the total naphthoquinone content in the effective part is determined by the UV direct method, and it is 65%; isofulvedin, shallotin and shallot A are determined by high-performance liquid chromatography, a...

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Abstract

The invention relates to the field of ethic drugs, and particularly relates to a preparation method of Eleutherine plicata Herb effective parts for treating coronary heart disease. The preparation method comprises the following steps: (1) primary extraction of Eleutherine plicata Herb effective parts: taking Eleutherine plicata Herb, adding an extracting solvent, and performing soaking, reflux, filtering, filtrate combination, concentration, to obtain a primary extracting liquid; and (2) purification of primary extracting liquid: filtering the primary extracting liquid and regulating the pH value, loading samples and performing dynamic adsorption with a macroporous adsorption resin column, washing to be neutral with water, dynamically parsing the Eleutherine plicata Herb effective parts, adsorbed by the macroporous adsorption resin column, with a parsing agent, to obtain a desorbed solution, partially or completely removing the solvent in the desorbed solution, to obtain the Eleutherine plicata Herb effective parts. According to the preparation method, the use of a large-toxicity organic solvent is not needed, so that not only can the usage cost be lowered, but also the environmentprotection is achieved; ethanol is selected as a parsing agent, thus being convenient to recycle, and non-toxic and environment-friendly; and the whole preparation process is economic and pollution-free, and suitable for industrial mass production.

Description

technical field [0001] The invention relates to the field of ethnic medicines, in particular to a preparation method and application of effective parts of small red garlic for treating coronary heart disease. Background technique [0002] Coronary atherosclerotic heart disease (CHD) is a cardiovascular disease known as "the number one killer of human health" and has become the number one cause of death in China. With the progress of society, human labor and dietary structure have changed, and many pathogenic factors such as high blood pressure, high blood fat, smoking, unreasonable diet, diabetes, obesity, and mental stress have more and more affected human physical and mental health. The incidence of coronary heart disease and its risk factors are increasing year by year, and they are getting younger. [0003] Ethnic medicine has a good prospect in the treatment of coronary heart disease. Dai medicine believes that the pathogenesis of coronary heart disease is the disorde...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/88A61P9/10
CPCA61K36/88A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 邱明丰唐雪澜苏靖马欣李心蕊
Owner SHANGHAI JIAO TONG UNIV
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