A method for preparing high-purity chlorogenic acid from chrysanthemum notoginseng

A technology of chrysanthemum notoginseng and chlorogenic acid is applied in the preparation of carboxylate, chemical instruments and methods, preparation of organic compounds, etc. Production, low purity of chlorogenic acid, etc., to achieve the effect of good retention time, high recovery rate and high monomer purity

Active Publication Date: 2020-10-09
江西省蔓三七健康科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

When these methods are used alone, the total yield of chlorogenic acid is about 2.5%, and the purity is below 40%, and it is difficult to achieve a purity of more than 90%.
At present, the production of chlorogenic acid mainly has the following problems: the method capable of extracting high-purity chlorogenic acid is not suitable for industrialized large-scale production, and the purity of chlorogenic acid obtained by the method suitable for industrialized large-scale production is low, which cannot meet Market demand for high-purity chlorogenic acid

Method used

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  • A method for preparing high-purity chlorogenic acid from chrysanthemum notoginseng
  • A method for preparing high-purity chlorogenic acid from chrysanthemum notoginseng

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Embodiment 1

[0023] A method for preparing high-purity chlorogenic acid from chrysanthemum notoginseng, comprising the following specific steps:

[0024] Step 1. Cut the flat lying chrysanthemum notoginseng leaves according to the length of 30mm to 40mm, and then place them in a constant temperature drying oven at 40°C for 40 minutes. After drying, the water content of the flat lying chrysanthemum notoginseng leaves is kept at 55% of the initial weight, and Carry out pulverization, cross 80 mesh sieves, obtain flat lying chrysanthemum notoginseng powder, pack for subsequent use;

[0025] Step 2. Weigh 100g of flat lying chrysanthemum notoginseng leaf powder and add it to 2000ml of methanol aqueous solution with a concentration of 65% by volume, and put it into an ultrasonic cleaner. The extraction temperature is 60°C, the extraction time is 40min, and the extraction power is 450W. The number of extractions is 3 times, filtered to obtain the extract, which is the crude extract of chlorogeni...

Embodiment 2

[0029] A method for preparing high-purity chlorogenic acid from chrysanthemum notoginseng, comprising the following specific steps:

[0030] Step 1. Cut the flat lying chrysanthemum notoginseng leaves according to the length of 30mm to 40mm, and then place them in a constant temperature drying oven at 50°C for 34 minutes. Pulverize and cross a 60-mesh sieve to obtain the flat lying chrysanthemum notoginseng powder, which is bagged for subsequent use;

[0031] Step 2. Weigh 100g of flat lying chrysanthemum notoginseng leaf powder and add it to 4000ml of methanol aqueous solution with a volume percentage concentration of 70%, and put it into an ultrasonic cleaner. The extraction temperature is 50°C, the extraction time is 30min, and the extraction power is 400W. The number of extractions is 3 times, filtered to obtain the extract, which is the crude extract of chlorogenic acid, and the extraction rate of chlorogenic acid is 3.58%;

[0032] Step 3, dilute the chlorogenic acid refe...

Embodiment 3

[0035] A method for preparing high-purity chlorogenic acid from chrysanthemum notoginseng, comprising the following specific steps:

[0036] Step 1. Cut the flat lying chrysanthemum notoginseng leaves according to the length of 30mm to 40mm, and then place them in a constant temperature drying oven at 60°C for 30 minutes. After drying, the water content of the flat lying chrysanthemum notoginseng leaves is kept at 70% of the initial weight. Pulverize and cross a 40-mesh sieve to obtain the flat lying chrysanthemum notoginseng powder, which is bagged for subsequent use;

[0037] Step 2. Weigh 100g of flat lying chrysanthemum notoginseng leaf powder and add it to 5000ml of methanol aqueous solution with a concentration of 75% by volume, and put it into an ultrasonic cleaner. The extraction temperature is 75°C, the extraction time is 30min, and the extraction power is 600W. The number of extractions is 5 times, filtered to obtain the extract, which is the crude extract of chlorog...

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Abstract

The invention discloses a method for preparing high-purity chlorogenic acid from gynura procumbens. The method includes the steps: taking leaves of the gynura procumbens as a raw material; cutting, drying, crushing the leaves of the gynura procumbens, and sieving the crushed leaves to obtain powder of the leaves of the gynura procumbens; performing microwave assisted extraction on the powder of the leaves of the gynura procumbens to obtain crude extracts of the chlorogenic acid; measuring the content of the chlorogenic acid in the crude extracts of the chlorogenic acid by a high-performance liquid chromatography; separating and purifying the crude extracts of the chlorogenic acid by a middle-high pressure preparation liquid chromatography to obtain a chlorogenic acid monomer with high purity and recovery rate. The method is high in separating efficiency and treatment capacity, short in production period and good in analysis method repeatability and provides effective technical supportfor industrial production of the high-purity chlorogenic acid in the gynura procumbens.

Description

technical field [0001] The invention belongs to the technical field of chlorogenic acid preparation, and in particular relates to a method for preparing high-purity chlorogenic acid from chrysanthemum notoginseng. Background technique [0002] Gynura procumbens (Lour.) Merr. is a genus of Panax notoginseng in the family Asteraceae, also known as snake bone and fairy grass. It is a perennial herb with the same source of medicine and food. Climbing on shrubs or trees, it is widely distributed in southern my country. In May 2012, Pingwo chrysanthemum notoginseng was approved by the Ministry of Health as a new resource food. Pingwo chrysanthemum notoginseng has a wide range of pharmacological effects. According to the second volume of "Compilation of Chinese Herbal Medicine", its curative effect is sweet and light, flat in nature, dredging meridian and activating collaterals, reducing inflammation and relieving cough, dissipating blood stasis and reducing swelling, promoting blo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C69/732C07C67/48C07C67/56C07C67/54
CPCC07C67/48C07C67/54C07C67/56C07C69/732
Inventor 付建平胡居吾吴磊熊伟王慧宾韩晓丹
Owner 江西省蔓三七健康科技有限公司
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