Cross-linked starch biosynthesis recombinant gene YXI and application thereof

Abstract

The invention discloses a cross-linked starch biosynthesis recombinant gene YXI and application thereof. The nucleotide sequence is shown as SEQ ID NO:1, glgC55 of the ADP-glucose pyrophosphorylase coding gene is separated out from a mutant strain of escherichia coli JM109-3, and an ADP-glucose pyrophosphorylase (AGPase) small subunit coding gene (SSU cDNA) and a starch grain-combined starch synthase coding gene GBSSI promoter are respectively separated out from potatoes. An AGPase subunit composed of 431 amino acids is coded by glgC55, wherein four specific mutations, which are K39E, V71A, I248V and K304E, exist, the first two appear in the Rossmann fold regions of the oligomeric subunit N-terminal metabolic groups, and the latter two appear in oligomeric subunit C-terminal heterogeneously regulated and oligomerized LbH groups.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and discloses a cross-linked starch biosynthesis recombinant gene YXI and its application. Background technique [0002] Starch is one of the most common and cheapest photopolymerizable carriers next to cellulose. Starch has a single composition, is easy to store, and has a wide range of uses. It is an important industrial raw material for building materials, textiles, papermaking, and oil extraction. In particular, the chemical energy stored in it is easy to convert and utilize again. For this reason, a new understanding of starch at a strategic level is in the ascendant. [0003] Starch comes from plant seeds (such as rice, wheat, corn, etc.), tubers (such as potatoes, etc.), etc. Due to food composition and starch characteristics, potato starch is a widely accepted and used industrial raw material; the starch yield of potato production areas in my country (The amount of tubers required to p...

AI Technical Summary

Problems solved by technology

[0020] AGPase is not only regulated by drought and other factors, but also the binding state of AGPase subunits is regulated by redox according to different photoperiods, and the expression of transcripts of large and small subunits is also differentiated; at present, the regulation of its activity and starch accumulation by AGPase itself is still in the small and large subunits However, its sensitivity to the environment and metabolic level determines that it cannot be a target gene for starch accumulation at present, and replacing endogenous AGPase with a non-sensitive gene is a feasible option at this stage

[0021] The isoform of the large and small subunits in the composition of endogenous AGPase is encoded by multiple genes, and it is still in the stage of its identification and function research. It is not suitable to use the isoform of the large and small subunits to regulate starch accumulation.

Images