Difunctional 5'-tri-phosphate siGPC-3, preparation method and application thereof

A dual-function and reactive technology, applied in the field of genetic engineering to achieve the effect of inhibiting proliferation and promoting apoptosis

Active Publication Date: 2018-03-16
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] So far, GPC3 can be used as a target for the treatment of liver cancer, and RNAi

Method used

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  • Difunctional 5'-tri-phosphate siGPC-3, preparation method and application thereof
  • Difunctional 5'-tri-phosphate siGPC-3, preparation method and application thereof
  • Difunctional 5'-tri-phosphate siGPC-3, preparation method and application thereof

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Embodiment Construction

[0033] It should be pointed out that the following detailed description is exemplary and intended to provide further explanation to the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

[0034] It should be noted that the terminology used here is only for describing specific implementations, and is not intended to limit the exemplary implementations according to the present application. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and / or "comprising" are used in this specification, they mean There are features, steps, operations, means, components and / or combinations thereof.

[0035] As introduced in the background technology, it has not been reported that GPC3 can be used as a target fo...

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Abstract

The invention discloses a difunctional 5'-tri-phosphate siGPC-3, a preparation method and application thereof. The difunctional 5'-tri-phosphate siGPC-3 is GPC-3 specific siRNA containing a triphosphate group, the sequence of a sense strand from 5' to 3' is CCUUGCAGAACUGGCCUAU, wherein the 5' end is modified with a triphosphate group, the sequence of an antisense strand from 5' to 3' is AUAGGCCAGUUCUGCAAGG, and the 5' end of the antisense strand is modified with a triphosphate group. The difunctional 5'-tri-phosphate siGPC-3 has both gene silencing function and RIG-I activation function.

Description

technical field [0001] The invention belongs to the field of genetic engineering and relates to the field of RNA interference (RNAi) technology treatment, in particular to a bifunctional 5'-tri-phosphate siGPC-3 and its preparation method and application. Background technique [0002] Liver cancer is one of the most common malignant tumors in the world, and its morbidity and mortality are increasing year by year, seriously threatening human health. The treatment of liver cancer mainly includes liver transplantation, tumor resection and non-resective local therapy such as hepatic arterial chemoembolization. However, because liver cancer, especially hepatocellular carcinoma (HCC), is prone to metastasis in the early stage and easy to relapse after treatment, the effect of the above-mentioned treatment methods in the treatment of hepatocellular carcinoma is not obvious. The therapeutic target of liver cancer is of great significance. [0003] Glypican 3 (glypican3, GPC3) is o...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/10A61K31/7088A61P35/00
CPCA61K31/7088C12N15/113C12N2330/50C12N2310/14Y02A50/30
Inventor 韩秋菊张彩于馨邵利伟张建
Owner SHANDONG UNIV
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