Improved mycobacterium staining solution and staining method
A technology of mycobacterium and dyeing solution, which is applied in the field of medicine, can solve the problems of low positive rate and inability to color well and firmly, and achieve the effect of easy commercialization, high application added value, and bright colors
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Embodiment 1
[0031] The invention provides an improved mycobacterium staining solution, which is configured according to the following ratio:
[0032] ① Oxidizing solution: 3% H 2 o 2 the solution
[0033] ② Primary dye solution: basic fuchsin 5.0g
[0034] Ethanol 400mL
[0035] Distilled water 600mL
[0036] DISPERBYK-2200 20mL
[0037] ③Decolorization solution: 5% hydrochloric acid ethanol (95%) solution
[0038] ④ counterstaining solution: methylene blue 6.0g
[0039] Ethanol 200mL
[0040] Distilled water 800mL
[0041] KOH 0.05g.
Embodiment 2
[0043] The invention provides an improved mycobacterium staining solution, which is configured according to the following ratio:
[0044] ① Oxidizing solution: 5% H 2 o 2 the solution
[0045] ② Primary dye solution: basic fuchsin 3.0g
[0046] Ethanol 250mL
[0047] Distilled water 700mL
[0048] DISPERBYK-2200 50mL
[0049] ③ Decolorization solution: 3% hydrochloric acid ethanol (95%) solution
[0050] ④Counterstain solution: 4.0g methylene blue
[0051] Ethanol 300mL
[0052] Distilled water 700mL
[0053] KOH 0.10g.
Embodiment 3
[0055] The invention provides an improved mycobacterium staining solution, which is configured according to the following ratio:
[0056] ① Oxidizing solution: 5% H 2 o 2 the solution
[0057] ② Primary dye solution: basic fuchsin 3.0g
[0058] Ethanol 250mL
[0059] Distilled water 700mL
[0060] DISPERBYK-180 50mL
[0061] ③ Decolorization solution: 3% hydrochloric acid ethanol (95%) solution
[0062] ④Counterstain solution: 4.0g methylene blue
[0063] Ethanol 300mL
[0064] Distilled water 700mL
[0065] KOH 0.10g.
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