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Improved mycobacterium staining solution and staining method

A technology of mycobacterium and dyeing solution, which is applied in the field of medicine, can solve the problems of low positive rate and inability to color well and firmly, and achieve the effect of easy commercialization, high application added value, and bright colors

Active Publication Date: 2018-03-27
CHENGDU RICH SCI IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional Ziehi-Neelsen acid-fast staining method is precisely because of the wax barrier of acid-fast bacteria, even if it is heated or prolonged, it still cannot be stained well and firmly, resulting in a low positive rate

Method used

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  • Improved mycobacterium staining solution and staining method
  • Improved mycobacterium staining solution and staining method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The invention provides an improved mycobacterium staining solution, which is configured according to the following ratio:

[0032] ① Oxidizing solution: 3% H 2 o 2 the solution

[0033] ② Primary dye solution: basic fuchsin 5.0g

[0034] Ethanol 400mL

[0035] Distilled water 600mL

[0036] DISPERBYK-2200 20mL

[0037] ③Decolorization solution: 5% hydrochloric acid ethanol (95%) solution

[0038] ④ counterstaining solution: methylene blue 6.0g

[0039] Ethanol 200mL

[0040] Distilled water 800mL

[0041] KOH 0.05g.

Embodiment 2

[0043] The invention provides an improved mycobacterium staining solution, which is configured according to the following ratio:

[0044] ① Oxidizing solution: 5% H 2 o 2 the solution

[0045] ② Primary dye solution: basic fuchsin 3.0g

[0046] Ethanol 250mL

[0047] Distilled water 700mL

[0048] DISPERBYK-2200 50mL

[0049] ③ Decolorization solution: 3% hydrochloric acid ethanol (95%) solution

[0050] ④Counterstain solution: 4.0g methylene blue

[0051] Ethanol 300mL

[0052] Distilled water 700mL

[0053] KOH 0.10g.

Embodiment 3

[0055] The invention provides an improved mycobacterium staining solution, which is configured according to the following ratio:

[0056] ① Oxidizing solution: 5% H 2 o 2 the solution

[0057] ② Primary dye solution: basic fuchsin 3.0g

[0058] Ethanol 250mL

[0059] Distilled water 700mL

[0060] DISPERBYK-180 50mL

[0061] ③ Decolorization solution: 3% hydrochloric acid ethanol (95%) solution

[0062] ④Counterstain solution: 4.0g methylene blue

[0063] Ethanol 300mL

[0064] Distilled water 700mL

[0065] KOH 0.10g.

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Abstract

The invention discloses an improved mycobacterium staining solution. The staining solution is prepared from an oxidization solution, a primary staining solution, a de-coloring solution and a compoundstaining solution, wherein the oxidization solution adopts an H2O2 solution or an H5IO6 solution; the primary staining solution adopts an alkaline magenta solution. The improved mycobacterium stainingsolution has the advantages of non-fluorescent staining, relatively low cost, simplicity in operation and no harms to human bodies; after the oxidization solution is used for oxidizing, staining is carried, so that alkaline magenta is more firmly combined with acid-fast bacillus; a stained flake not only has a bright color, but also can be stored for long time.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an improved mycobacterium staining solution and a staining method. Background technique [0002] In recent years, due to the prevalence of acquired immunodeficiency syndrome (AIDS), the emergence of drug-resistant strains of Mycobacterium tuberculosis, the application of immunosuppressants, drug abuse, poverty and population mobility, the prevalence of tuberculosis has a tendency to come back in the world. Tuberculosis has become a major public health problem worldwide. Acid-fast staining of diseased tissues and finding acid-fast stain-positive Mycobacterium tuberculosis is still one of the most effective and accurate methods for diagnosing tuberculosis. However, the traditional Ziehi-Neelsen antibacterial The operation of acid staining method is quite different in different hospitals, and the positive rate is low, which has caused certain difficulties in diagnosis. In the 1880...

Claims

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Application Information

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IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 张力
Owner CHENGDU RICH SCI IND