Efficient seedling hardening technology and domestication cultivation method for longleaf campanumoea tissue culture seedling

A cultivation method and technology of red ginseng are applied in the field of efficient hardening and domestication of red ginseng tissue culture seedlings, and can solve the problems of slow growth of seedlings, large variation of offspring, and difficulty in large-scale promotion in large areas.

Inactive Publication Date: 2018-04-03
丽江市古城区秋成种养殖有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Red fruit ginseng uses seeds as the reproductive unit in the wild, and its seedlings grow slowly and the offspring vary greatly, and are limited by the germinati

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Select good red ginseng plants without diseases and insects, rinse with flowing water, soak in 3% lime water for 10 minutes for disinfection, remove the side buds and disinfect them with chlorine dioxide on an ultra-clean workbench, and then inoculate In the aseptic wort medium, shake culture to obtain the red fruit ginseng tissue culture seedling; the red fruit ginseng tissue culture seedling is moved to red loam 100Kg, sandy soil 80Kg and the mass ratio of nitrogen, phosphorus and potassium is the nitrogen phosphorus of 10: 3: 3 Carry out seedling hardening and cultivate 3 months on the seedbed of potash fertilizer 30Kg to obtain the cultivated seedling of red ginseng, and the survival rate is 98%; Cultivate in an environment of 80-90% for 20 days, then gradually adjust the light intensity to 5000-6000Lx within 1.5 months, and the humidity to 55-65%, and continue to domesticate and cultivate for 2 months to obtain domesticated seedlings of red fruit ginseng with a surv...

Embodiment 2

[0023] Select the excellent red ginseng plants without disease and pests, rinse with flowing water, soak in 1% lime water for 10 minutes for disinfection, remove the side buds and disinfect them with chlorine dioxide on the ultra-clean workbench, and then inoculate In the aseptic wort medium, shake culture to obtain the red fruit ginseng tissue culture seedling; the red fruit ginseng tissue culture seedling is moved to red loam 50Kg, sandy soil 40Kg and the mass ratio of nitrogen, phosphorus and potassium is the nitrogen phosphorus of 8: 1: 1 Carry out seedling hardening on the seedbed of potash fertilizer 20Kg and cultivate for 1 month to obtain the cultivated seedlings of red ginseng, and the survival rate is 96%; the cultivated seedlings of red ginseng are transplanted to the seedbed of red loam for domestication and cultivation. Cultivate in an environment of 70-80% for 20 days, then gradually adjust the light intensity to 3000-5000Lx and the humidity to 55-65% within 0.5 m...

Embodiment 3

[0025] Select excellent red ginseng plants without disease and pests, rinse with flowing water, soak in 2% lime water for 30 minutes for disinfection, remove the side buds and disinfect them with chlorine dioxide on an ultra-clean workbench, and then inoculate Shake culture in sterile wort medium to obtain red fruit ginseng tissue culture seedlings; red fruit ginseng tissue culture seedlings are moved to red loam 100Kg, sandy soil 40Kg and nitrogen, phosphorus and potassium in a mass ratio of 9:2:2. Carry out seedling hardening and cultivate 2 months on the seedbed of potassium fertilizer 25Kg to obtain the cultivated seedlings of red ginseng, and the survival rate is 99%; Cultivate in an environment of 70-80% for 30 days, then gradually adjust the light intensity to 5000-6000Lx within 1 month, and the humidity to 55-65%, and continue to domesticate and cultivate for 1.5 months to obtain domesticated seedlings of red fruit ginseng with a survival rate of 99%. %.

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Abstract

The invention discloses an efficient seedling hardening technology and a domestication cultivation method for a longleaf campanumoea tissue culture seedling. The efficient seedling hardening technology for the longleaf campanumoea tissue culture seedling comprises the following steps: selecting a high-quality disease-free and insect pest-free longleaf campanumoea plant for bleaching and disinfection, and extracting lateral buds for sterilization and disinfection, and then are inoculated into a sterile liquid culture medium to obtain the longleaf campanumoea tissue culture seedling; transferring the longleaf campanumoea tissue culture seedling onto a seedling bed of a seedling hardening substrate, and performing seedling hardening culture for 1 to 3 months to obtain a longleaf campanumoea cultivated seedling. The domestication cultivation method for the longleaf campanumoea cultivated seedling comprises the following steps: transplanting the longleaf campanumoea cultivated seedling ontoa seedling bed of red soil, performing cultivation for 20 to 40 days in an environment with illuminance of 1,000 to 1,500Lx and humidity of 70 to 99 percent, then gradually regulating the illuminanceto 3,000 to 6,000Lx and regulating the humidity to 55 to 65 percent in a time range of 0.5 to 1.5 months, and continuing domestication cultivation for 1 to 2 months to obtain a longleaf campanumoea seedling. The survival rate of the longleaf campanumoea tissue culture seedling obtained by the efficient seedling hardening technology for the longleaf campanumoea tissue culture seedling is 95 percent or more, and the survival rate of the domestication cultivation method for the longleaf campanumoea cultivated seedling is 97 percent or more.

Description

technical field [0001] The invention belongs to the technical field of planting, and in particular relates to a high-efficiency seedling hardening technology and a domestication cultivation method of red fruit ginseng tissue culture seedlings. Background technique [0002] Red fruit ginseng, perennial erect or trailing herb. Root carrot-shaped. Usually the whole plant is glabrous, hollow, with many and long branches, flat or drooping. The leaves are opposite, occasionally 3 whorls, and short-stalked; the leaves are ovate, ovate-lanceolate to lanceolate. Flowers are usually single, terminal and axillary; pedicel or peduncle; a pair of filamentous bracteoles in the upper part of the pedicel or at the base of the drug; calyx is only attached to the lower part of the ovary, and the lobes are usually 5, far away from each other, filamentous Or strip-shaped, with branched slender teeth at the edge; corolla white or light red, tubular bell-shaped; stamens 5-6, filaments and anth...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G22/25
CPCA01H4/008
Inventor 李文忠王美蓉
Owner 丽江市古城区秋成种养殖有限公司
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