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A method for inducing callus by hemp seeds

A technology of callus and hemp, which is applied in the field of hemp seed induction callus, can solve the problems that hinder the large-scale production of hemp, cannot meet the demand of hemp, and the low germination rate of seeds, so as to improve the sterilization effect, The effect of increasing germination rate and reducing pollution rate

Active Publication Date: 2021-04-13
BAISE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the breeding technology of hemp is mainly based on seed propagation, that is, sexual reproduction, which has disadvantages such as low seed germination rate, long germination time, inconsistent germination, and high possibility of variation. The above problems have seriously hindered the scale of hemp. The production cannot meet the market demand for hemp

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for inducing callus with hemp seeds, comprising the following steps:

[0022] (1) Pretreatment: select complete and plump hemp seeds of uniform size, wash them with running water, and use ultrasonic treatment with a power of 100W and a frequency of 30KHz for 20 minutes, and keep the hemp seeds soaked in the medium for 30 minutes. The medium for the first 10 minutes is water at 40 ° C, and the medium for 10 minutes after treatment is GGR solution with a mass concentration of 25 mg / L; put the hemp seeds after ultrasonic treatment into a petri dish covered with a damp cloth, and then cover with a layer of damp cloth After that, place it at 25°C for 8h;

[0023] (2) Disinfection treatment: after the above-mentioned pretreated hemp seeds are peeled off the seed coat, the hemp seeds are obtained, and after the described hemp seeds are rinsed with running water for 10 minutes, the volume fraction is 70% alcohol for 15 seconds, and then Rinse with sterile water for 3 ...

Embodiment 2

[0026] A method for inducing callus with hemp seeds, comprising the following steps:

[0027] (1) Pretreatment: select complete and plump hemp seeds of uniform size, wash them with running water, and use ultrasonic treatment with a power of 110W and a frequency of 30KHz for 20 minutes, and keep the hemp seeds soaked in the medium for 45 minutes. The medium for the first 10 minutes is water at 43 ° C, and the medium for 10 minutes after treatment is GGR solution with a mass concentration of 32 mg / L; put the hemp seeds after ultrasonic treatment into a petri dish covered with a damp cloth, and then cover with a layer of damp cloth After that, place it at 25°C for 9h;

[0028] (2) Disinfection treatment: after the above-mentioned pretreated hemp seeds are peeled off the seed coat, the hemp seeds are obtained, and after the described hemp seeds are rinsed with running water for 15min, the volume fraction is 72% alcohol for 25s, and then Rinse with sterile water for 3 times, then ...

Embodiment 3

[0031] A method for inducing callus with hemp seeds, comprising the following steps:

[0032] (1) Pretreatment: select complete and plump hemp seeds of uniform size, wash them with running water, and use ultrasonic treatment with a power of 120W and a frequency of 30KHz for 20 minutes, and keep the hemp seeds soaked in the medium for 50 minutes. The medium for the first 10 minutes is water at 45 ° C, and the medium for 10 minutes after treatment is GGR solution with a mass concentration of 35 mg / L; put the hemp seeds after ultrasonic treatment into a petri dish covered with a damp cloth, and then cover with a layer of damp cloth After that, place it at 25°C for 10h;

[0033] (2) Disinfection treatment: peel off the seed coat of the above-mentioned hemp seeds through pretreatment to obtain the hemp seeds, rinse the hemp seeds with running water for 20 minutes, and treat them with 75% alcohol for 30 seconds with volume fraction, then use Rinse with sterile water for 3 times, th...

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Abstract

The invention belongs to the field of biotechnology, and specifically discloses a method for inducing callus with hemp seeds. The method for inducing callus of hemp seeds of the present invention comprises the following steps: (1) pretreatment: adopt ultrasonic wave to treat hemp seeds; (2) disinfection treatment: adopt alcohol and mercuric chloride to treat hemp seeds successively and peel off the seed coat (3) inoculation culture: inoculate the above-mentioned sterilized hemp seeds in the induction medium and cultivate them to obtain the hemp seed. The method for inducing callus by hemp seeds of the present invention utilizes hemp seeds as explants to carry out tissue culture to induce callus, the induction rate is high, and the quality of the induced callus is good, which is obtained by subsequent cultivation and differentiation of hemp seeds A large number of robust tissue culture seedlings provide the basis.

Description

【Technical field】 [0001] The invention relates to the field of biotechnology, in particular to a method for inducing callus with hemp seeds. 【Background technique】 [0002] Hemp, also known as thread hemp and sweat hemp, is an annual herb of the genus Hemp in the Moraceae family. After the hemp seed is peeled, it becomes the hemp seed, which contains a variety of nutrients, such as vitamin B, vitamin E, resin, volatile oil and other components. Hemp seed is rich in oil, its oil content is 30%, higher than that of soybean, unsaturated fatty acid accounts for 70%-80%, mainly linoleic acid, linolenic acid and oleic acid, the content is 0.61% respectively , 24.42%, 52.32%, wherein the ratio of linoleic acid and linolenic acid is close to 2.5, which is the best ratio for human body to absorb fatty acids. Among them, unsaturated fatty acids have anti-oxidation, anti-tumor, and immune-enhancing effects, and can significantly reduce the content of high-density protein serum choles...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01C1/00
CPCA01C1/00A01H4/001A01H4/008
Inventor 农艳丰李健朱德生
Owner BAISE UNIV
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