Application of stat1 promoter methylation detection in drug-resistant tumor cells
A technology of tumor cells and drug-resistant cells, which is applied in the field of biomedicine and can solve problems such as alisertib drug resistance that has not been monitored
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[0057] The application of STAT1 promoter methylation detection in drug-resistant tumor cells includes the following steps:
[0058] (1) Establishment and identification of drug-resistant cells in vitro: construct drug-resistant glioma U251 cells and colon cancer SW480 cells in vitro, culture glioma cells and colon cancer cells in a specific medium for 6 months, and then use MTT method was used to detect drug-resistant glioma cells and colon cancer cells; the special medium for glioma U251 cells was purchased from Cyagen;
[0059] The method for constructing drug-resistant glioma U251 cells is as follows:
[0060] After the U251 cells were cultured for 1 week in a special medium containing a final concentration of 1 nM alisertib, the surviving U251 cells were resuspended in a special medium with a final concentration of 10 nM alisertib to continue culturing. The dedicated medium containing alisertib at a final concentration of 10 nM was replaced every 2 days. After 3 weeks, t...
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