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Compound lactobacillus micro-ecological preparation and preparation method and application thereof

A technology of micro-ecological preparations and compound lactic acid bacteria, applied in the biological field, can solve problems such as the inability to realize multi-angle, all-round and multi-functional regulation of the intestinal flora structure of pet cats, and the poor system of a single strain, so as to optimize the mental state and Effects on coat condition, litter odor reduction, and improved feed intake and growth rate

Active Publication Date: 2018-04-27
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the above-mentioned technical problems, this application proposes a compound lactic acid bacteria microecological preparation and its preparation method and application to solve the problem of poor systemicity of a single strain and the inability to realize multi-angle, all-round and multi-functional regulation of the intestinal flora structure of pet cats. question

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  • Compound lactobacillus micro-ecological preparation and preparation method and application thereof
  • Compound lactobacillus micro-ecological preparation and preparation method and application thereof
  • Compound lactobacillus micro-ecological preparation and preparation method and application thereof

Examples

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Embodiment 1

[0046] Preparation of Lactobacillus plantarum LP6, Lactobacillus casei zhang, Lactobacillus plantarum P-8, Bifidobacterium animalis V9 and preparation of compound lactic acid bacteria microecological preparation

[0047] In step 1, Lactobacillus plantarum LP6, Lactobacillus casei, Lactobacillus plantarum and Bifidobacterium animalis are respectively subjected to primary fermentation to obtain corresponding primary seed culture solutions.

[0048] The fermentation conditions of the primary fermentation include: Lactobacillus plantarum LP6, Lactobacillus casei, Lactobacillus plantarum and animal Bifidobacterium respectively at the inoculation amount of 2%-10% (v / v), temperature 33-37°C, rotation speed 50-100r Cultivate for 18-24 hours under the condition of / m; preferably, cultivate for 20-22 hours under the conditions of inoculum size 5%-8% (v / v), temperature 35-36°C, and rotational speed 60-80r / m.

[0049] The effective number of viable bacteria of Lactobacillus plantarum LP6 ...

Embodiment 2

[0101] The preparation of embodiment 2 compound lactic acid bacteria probiotics

[0102] By the same method as in Example 1, these four strains of Lactobacillus plantarum LP6, Lactobacillus casei zhang, Lactobacillus plantarum P-8, and Bifidobacterium animalis V9 were respectively carried out to primary, secondary seed culture, and tertiary fermentation culture. Centrifuge separately to obtain the cells of these four strains of bacteria, mix the cells with the protective agent solution according to the mass ratio of 1:5, 1:8, 1:5, and 1:10 to obtain a bacterial suspension, and the bacterial suspension The solution was freeze-dried to obtain bacterial agents respectively; said Lactobacillus plantarum LP6 bacterial agent, Lactobacillus casei zhang bacterial agent, Lactobacillus plantarum P-8 bacterial agent, animal Bifidobacterium V9 bacterial agent in a ratio of 1:1:1 :2 to mix, and add the dilution carrier to compound, to obtain the compound lactic acid bacteria microecologica...

Embodiment 3

[0103] The preparation of embodiment 3 compound lactic acid bacteria probiotics

[0104] By the same method as in Example 1, these four strains of Lactobacillus plantarum LP6, Lactobacillus casei zhang, Lactobacillus plantarum P-8, and Bifidobacterium animalis V9 were respectively carried out to primary, secondary seed culture, and tertiary fermentation culture. Centrifuge separately to obtain the cells of these four strains of bacteria, mix the cells with the protective agent solution according to the mass ratio of 1:7, 1:10, 1:5, and 1:8 to obtain a bacterial suspension, and the bacterial suspension The solution was freeze-dried to obtain bacterial agents respectively; said Lactobacillus plantarum LP6 bacterial agent, Lactobacillus casei zhang bacterial agent, Lactobacillus plantarum P-8 bacterial agent, animal Bifidobacterium V9 bacterial agent in a ratio of 1:1:1 : 7 for mixing, and adding the dilution carrier to compound, to obtain the compound lactic acid bacteria microe...

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Abstract

The invention provides a compound lactobacillus micro-ecological preparation and a preparation method and application thereof. The compound lactobacillus micro-ecological preparation is prepared by mixed compounding of a lactobacillus plantarum LP6 bacterial agent, a Lactobacillus casei zhang bacterial agent, a lactobacillus plantarum P-8 bacterial agent, a bifidobacterium animalis V9 bacterial agent and a dilute carrier, wherein the viable count of each of the four bacterial agents is larger than or equal to 2*10<11>CFU / g. By application of the compound lactobacillus micro-ecological preparation with the viable count of 1*10<9>CFU / g to 30 different kinds of pet cats for 30 days on trail, the health level of the pet cats can be raised, for instance, the diarrhea incidence rate of the pet cats can be controlled effectively, mental conditions and fur conditions are optimized, hair loss is reduced, the ammonia concentration content of excrement is reduced, and cat litter odor is alleviated; in addition, the feed intake and growth rate are increased evidently, and the content of immune globulins G in blood of the pet cats and the content of secretory immunoglobulins A in excrement of the pet cats are remarkably increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a compound lactic acid bacteria microecological preparation and a preparation method and application thereof. Background technique [0002] With the improvement of people's living standards, family pet cats have gradually become popular and popular from the niche exclusive use of some high-income groups in the past. Foreign cat breeds such as cats, Persian cats, Balinese cats and fold-eared cats can bring beneficial effects to people of different ages. For example, feeding pet cats to children can cultivate their love, sense of responsibility and independent thinking ability from an early age Feeding pet cats for middle-aged people can release the pressure of work and life to a certain extent, and relax; feeding pet cats for the elderly can alleviate loneliness, enrich leisure life, and improve physical and mental happiness. [0003] As a small omnivorous animal, both do...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18A23K50/40C12R1/25C12R1/245C12R1/01
CPCA23K10/18A23K50/40C12N1/20
Inventor 黄卫强乔向前
Owner INNER MONGOLIA SCI PLUS BIOTECH
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