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A detection method and application of functional active ingredients of Spatholobus

A technology of active ingredients and detection methods, which is applied in the detection field of functional active ingredients of Spatholobus, can solve the defects of the quality control methods of Spatholobus, the inability to effectively control the quality of Spatholobus medicinal materials, and the quality control methods of Chinese patent medicines and other problems, to achieve the effect of good linear relationship, good precision and good repeatability

Active Publication Date: 2020-07-28
HUAIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Chinese Pharmacopoeia does not include the quality control methods and functional active ingredient detection indicators of S. sagelifolia, which cannot effectively control the quality of S. sagelifolia medicinal materials, and thus makes the quality control methods of Chinese patent medicines containing S.

Method used

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  • A detection method and application of functional active ingredients of Spatholobus
  • A detection method and application of functional active ingredients of Spatholobus
  • A detection method and application of functional active ingredients of Spatholobus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A method for detecting functional and effective ingredients of Sargentodoxa cuneata includes the following steps:

[0038] S1, prepare standard solutions of rutin, schisandrin A and schisandrin B respectively:

[0039] (1) Preparation of the rutin standard solution: accurately weigh out 0.0091g ​​of the rutin standard into a 10mL volumetric flask, add methanol to make the volume to 10mL, and obtain a 0.91mg / mL rutin standard solution.

[0040] (2) Preparation of schisandrin A standard solution: accurately weigh 0.0043 g of schisandrin A standard into a 10 mL volumetric flask, add methanol to a constant volume to 10 mL, and obtain a 0.43 mg / mL schisandrin A standard solution.

[0041] (3) Preparation of schisandrin B standard solution: accurately weigh out 0.0010 g of schisandrin B standard in a 10 mL volumetric flask, add methanol to make the volume to 10 mL, and obtain a 0.10 mg / mL schisandrin B standard solution.

[0042] S2, prepare the test solution

[0043] Accurately weigh 1...

Embodiment 2

[0048] A method for detecting functional active ingredients of Sargentodoxa cuneata. The steps are basically the same as those in Example 1, except that the mass of Sargentodoxa cuneata is 10.1235g in S2, 45ml of methanol is added, and the ultrasound time is 40min.

Embodiment 3

[0050] A method for detecting functional active ingredients of Sargentodoxa cuneata. The steps are basically the same as those in Example 1, except that the mass of Sargentodoxa cuneata is weighed in S2 as 10.4751 g, the methanol added is 55 ml, and the ultrasound time is 35 min.

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Abstract

The invention belongs to the technical field of the detection of components of traditional Chinese medicines, and discloses a detection method of effective components of radix seu caulis schisandrae propinquae and application. The detection method comprises steps of preparation of standard substance solutions of rutin, schizandrin A and schisandrin B respectively, preparation of a test sample solution, HPLC (High Performance Liquid Chromatography) detection and the like, wherein chromatographic conditions of the HPLC detection are as follows: a methanol-1 percent glacial acetic acid solution is used as mobile phase eluent; the gradient elution is carried out for 90min; volume fractions of methanol in the eluent corresponding to each time interval of the elution are respectively: 20 percentduring 0min to 20min, 40 percent during 20min to 40min, 60 percent during 40min to 60min, 80 percent during 60min to 80min and 100 percent during 80min to 90min; a flow speed is 1mL / min; a detectionwavelength is 257nm. The detection method uses the HPLC detection with the gradient elution, can be used for measuring three functional effective components in the radix seu caulis schisandrae propinquae at the same time, and can be used for more completely reflecting a pharmacodynamic effect of the radix seu caulis schisandrae propinquae.

Description

Technical field [0001] The invention belongs to the technical field of detection of components of traditional Chinese medicine, and specifically relates to a detection method and application of the functional and effective components of Sargentodoxa cuneata. Background technique [0002] Small blood vine (Schisandra Propinqua (Wall.) Baill. Var. Sinensis Oliv., Dchisandrapropingua var. sinensis), also known as Tieguasan, is a deciduous woody liana of the genus Schisandra of the Magnoliaceae. Its roots, canes and leaves are used as medicine. It is reported in the literature that Sargentodoxa cuneata mainly contains epienshixin, enshicine, isoschizandrolic acid, deoxyschizandrin, β-sitosterol, and stearic acid. And other chemical ingredients. We found that it contains more rutin, schisandrin A and schisandrin B by thin layer chromatography. Rutin, schisandrin A, and schisandrin B are the effective ingredients with definite efficacy. They are the quality control indicators of man...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 赵子剑赵畅梁玉兰诸葛树彬刘小波严丽莹王斌
Owner HUAIHUA UNIV
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