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Detection method and culture medium of Bacillus coagulans

A technology of Bacillus coagulans and culture medium, which is applied in the field of culture medium, Bacillus coagulans detection method and culture medium, can solve the problems of no detection method, etc., and achieve comprehensive detection methods, accurate and reliable detection results, and strong operability Effect

Active Publication Date: 2021-11-23
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is neither a national standard method nor a feasible detection method at present

Method used

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  • Detection method and culture medium of Bacillus coagulans
  • Detection method and culture medium of Bacillus coagulans
  • Detection method and culture medium of Bacillus coagulans

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0079] 4.1 Sample preparation and dilution

[0080] Operation process:

[0081] (1) Solid and semi-solid samples: Weigh 25g of the sample and place it in a sterile homogeneous bag filled with 225mL of phosphate buffer or normal saline diluent, beat it with a slap-type homogenizer for 1min to 2min, and make 1: 10 sample homogenate.

[0082] (2) Liquid sample: Take 25mL sample with a sterile pipette and put it into a sterile Erlenmeyer flask filled with 225mL phosphate buffer saline or normal saline (an appropriate number of sterile glass beads are preset in the bottle), mix well, and prepare Make a 1:10 sample homogeneous solution.

[0083] (3) Use a sterile pipette to draw 5mL-10mL of the primary dilution of the liquid test sample, solid or semi-solid test sample into a sterile test tube, and immediately put the above test tube in a water bath at 80°C ± 2°C, (the liquid in the test tube surface at least 4cm below the water surface) heat treatment for 30min ± 1min. Place a ...

Embodiment 1

[0118] Count detection of added Bacillus coagulans sample

[0119] 1. Equipment and materials

[0120] Ultra-clean bench or biological safety cabinet;

[0121] Autoclave;

[0122] Homogenizer;

[0123] Electronic balance (sensitivity is 0.1g);

[0124] Constant temperature incubator: 30℃±1℃;

[0125] Refrigerator: 2℃~5℃;

[0126] Graduated pipette: 1mL (with 0.01mL scale), 10mL (with 0.1mL) scale or micropipette and tip;

[0127] Triangular flask: 500m mL, 1000mL;

[0128] Test tube: 18×180;

[0129] Water bath: 80°C ± 1°C;

[0130] Sterile homogeneous bag.

[0131] 2. Media reagents

[0132] 2.1 Bacillus coagulans count medium

[0133] The preparation method of culture medium:

[0134] Ingredients: Yeast extract 2.5g, peptone 5.0g, glucose 1.0g, polysorbate 80 1.0g, L-cysteine ​​0.1g, bromocresol violet 0.06g, agar 15.0g. pH=7.0

[0135] Subpackaging: Add the above ingredients into 1000mL distilled water, heat to dissolve, and autoclave at 121°C for 15-20min aft...

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Abstract

The invention relates to a detection method and culture medium for bacillus coagulans. The medium includes: 5.0-10.0 parts by weight of tryptone, 2.0-3.0 parts by weight of yeast extract powder / paste, 1.0-5.0 parts by weight of glucose, 1.0-2.0 parts by weight of polysorbate-80, and 0.1-3.0 parts by weight of L-cysteine 0.5 parts by weight, 0.04-0.06 parts by weight of bromocresol violet, and 15.0-20.0 parts by weight of agar. The culture medium can make the colony grow well, the cost is low, the preparation is simple and convenient, and the counting result is accurate and credible when the culture medium is used to detect the bacillus coagulans.

Description

technical field [0001] The invention relates to the field of food, in particular, the invention relates to a culture medium and a detection method and culture medium for Bacillus coagulans. Background technique [0002] At present, Bacillus coagulans is widely used as a beneficial bacterium, such as being added to food or animal feed. Therefore, the importance and necessity of quantitative counting of Bacillus coagulans has become increasingly prominent. However, there is neither a national standard method nor a feasible detection method at present. [0003] Therefore, effective and feasible methods for detecting Bacillus coagulans are in urgent need of research and development. Contents of the invention [0004] This application is based on the inventor's discovery and recognition of the following facts and problems: [0005] The importance and necessity of quantitative counting of Bacillus coagulans has become increasingly prominent. However, there is neither a nationa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/06C12R1/07
CPCC12N1/20C12Q1/06
Inventor 薛志清吴玉秋李志国许璐袁凤琴喻东威常建军宋晓东
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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