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Method for researching anti-fatigue action of oligopeptides of Apostichopus japonicus Liao on mice

A technology of oligopeptide and Apostichopus japonicus, applied in the field of biotechnology, can solve problems such as limited research

Inactive Publication Date: 2018-05-25
大连平岛天然产物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the research on sea cucumber oligopeptides is limited

Method used

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  • Method for researching anti-fatigue action of oligopeptides of Apostichopus japonicus Liao on mice
  • Method for researching anti-fatigue action of oligopeptides of Apostichopus japonicus Liao on mice
  • Method for researching anti-fatigue action of oligopeptides of Apostichopus japonicus Liao on mice

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Embodiment Construction

[0023] The embodiments of the present invention will be further described below in conjunction with specific examples.

[0024] The sample in this example is Liao japonicus oligopeptide: the molecular weight obtained by Liao japonicus oligopeptide after desalting, chopping, and refining, adding compound protease to enzymatically hydrolyze, and then separating by decolorization, deodorization, and deionization. Peptide powder below 1000 Daltons; soybean oligopeptides: Peptide powder with a molecular weight below 1000 Daltons obtained by enzymatically hydrolyzing non-genetically modified edible soybean meal with complex proteases and then debittering and deodorizing. The above two oligopeptides are all provided by Dalian Haoxiang Bioenzyme Engineering Co., Ltd.; Liao sea cucumber powder: take dried Liao sea cucumber, pulverize, and pass through a 60-mesh sieve. Experimental animals are: healthy Kunming mice, weighing 20±2g, all male, purchased from Dalian Medical University. In...

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Abstract

The invention discloses a method for researching the anti-fatigue action of oligopeptides of Apostichopus japonicus Liao on mice and relates to the technical field of biology. Detecting the molecularweight of oligopeptides of the Apostichopus japonicus Liao firstly; performing grouping for an experiment and selecting gavage dose: 240 male Kunming mice weighing 20 g plus / minus 2 g are selected anddivided randomly into 8 groups, 30 mice are contained in each group in which 10 mice are used for loaded swimming, 10 mice are used for determination of serum urea nitrogen and 10 mice are used for determination of hepatic glycogen; performing a loaded swimming experiment; determining serum urea nitrogen; determining hepatic glycogen: 30 min after last gavage, the mice are killed with cervical dislocation, and livers are rinsed with normal saline, dried by filter paper and determined; after deep processing such as enzymolysis and the like on the Apostichopus japonicus Liao, oligopeptides withthe molecular weight smaller than 1000 Da are obtained. The anti-fatigue degree of oligopeptides of the Apostichopus japonicus Liao is compared with anti-fatigue degrees of soybean oligopeptides andApostichopus japonicus Liao powder, scientific basis is provided for access of oligopeptides of the Apostichopus japonicus Liao to market, and foundation is laid for development, utilization and popularization of novel marine drug resources.

Description

Technical field: [0001] The invention relates to a method for researching the anti-fatigue effect of japonicus japonicus oligopeptide on mice, and belongs to the technical field of biotechnology. Background technique: [0002] There are more than 1,400 species of sea cucumbers in the world, which are distributed in the vast sea areas of the intertidal zone and the ocean floor, among which the tropical sea area is rich in species. Sea cucumber is distributed in the sea area north of Lianyungang, Jiangsu Province, China, and it is the best quality among more than 20 kinds of edible sea cucumbers in China. Among them, Liao sea cucumber is considered to be a traditional Chinese medicine tonic and the best among sea cucumbers. As early as in the "Food Materia Medica" of the Ming Dynasty, it was pointed out that sea cucumbers have the health-preserving functions of nourishing vitality, nourishing the internal organs and eliminating deficiency. [0003] Many studies on the health...

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Application Information

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IPC IPC(8): A61K38/01A61P39/00G01N30/02G01N33/48
CPCA61K38/012G01N30/02G01N33/48
Inventor 黄祥贤
Owner 大连平岛天然产物科技有限公司
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