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Application of protein signal transduction regulatory factor 14 in the preparation of drugs for the treatment of fatty liver and related diseases

A technology of signal transduction and regulation factor, applied in the field of gene function and application, to achieve high safety effect

Active Publication Date: 2020-06-09
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of RGS14 in fatty liver has not yet been reported

Method used

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  • Application of protein signal transduction regulatory factor 14 in the preparation of drugs for the treatment of fatty liver and related diseases
  • Application of protein signal transduction regulatory factor 14 in the preparation of drugs for the treatment of fatty liver and related diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] [Example 1] The effect of RGS14 overexpression on fat accumulation in liver cells

[0087] Construct a lentiviral expression vector overexpressing RGS14, transfect HEK-293T cells, package lentivirus, and infect L02 cells to construct a stable cell line overexpressing RGS14 (pHAGE-RGS14), and overexpression empty vector as a control group (pHAGE group) , By Western blot to detect whether the stable cell line expresses RGS14 highly; the successfully expressed L02 cell seed plate is divided into 4 groups, namely pHAGE control group, pHAGE-RGS14 control group, pHAGE experimental group, pHAGE-RGS14 experimental group. After the cells were attached, the experimental group was stimulated with palmitate (PA, final concentration 0.4mM) and oleic acid (OA, 0.8mM), while the control group was added with the same amount of BSA, and oil red O staining was performed 16 hours later.

[0088] Western blot test results such as figure 1 As shown, the protein expression level of L02 cell line ...

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Abstract

The invention discloses an application of a protein signal transduction regulatory factor 14 in the preparation of drugs for treating fatty liver and related diseases, and belongs to new uses of RGS14. A result of the research of the functions of an RGS14 gene through a palmitate (PA) and oleic acid (OA) stimulus-induced liver cell lipid accumulation model with a human normal liver L02 cell line and an RSG14 over-expression L02 cell line as research objects shows that the RSG14 over-expression can significantly improve the lipid accumulation in liver cells, so the RGS14 can protect the liver cells from being subjected to lipoid denaturation. The RGS14 is a body endogenous protein, so the RGS14 has an excellent safety when used as a drug.

Description

Technical field [0001] The present invention belongs to the field of gene function and application, and particularly relates to the function and application of regulator of G protein signaling 14 (RGS14) gene in fatty liver and related diseases, and the preparation of RGS14 as a target gene Application in medicine for preventing, relieving and / or treating fatty liver and related diseases. Background technique [0002] Non-alcoholic fatty liver disease (NAFLD) is a metabolic disease that has no history of excessive drinking and is characterized by fat accumulation and degeneration of liver cells. Its pathogenic factors include central obesity, insulin resistance, and blood lipids. Abnormalities and metabolic syndrome, etc. [1-3] . NAFLD is the most common chronic liver disease. According to global statistics, the incidence of NAFLD is about 25%, and 10-20% of patients eventually develop non-alcoholic steatohepatitis (NASH). About one-third of NASH patients gradually evolve into ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/19A61K48/00A61P1/16
CPCA61K38/19A61K48/005
Inventor 李红良胡宇峰
Owner WUHAN UNIV
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