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Application of long non-coding RNA LINC00842 as a biomarker in the preparation of prognostic detection preparations for lung adenocarcinoma

A long-chain non-coding, lung adenocarcinoma technology, applied in the field of tumor molecular biology, can solve the problems of being unable to adapt to the prognosis of lung adenocarcinoma patients, and having no reference standards and no specific indicators for the prognosis of lung adenocarcinoma patients. Achieve far-reaching clinical significance and promotional effects

Active Publication Date: 2021-11-05
XIANGYA HOSPITAL CENT SOUTH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no reference standard for the prognosis of patients with lung adenocarcinoma, and there is no specific index, which is far from meeting the needs of the prognosis of patients with lung adenocarcinoma

Method used

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  • Application of long non-coding RNA LINC00842 as a biomarker in the preparation of prognostic detection preparations for lung adenocarcinoma
  • Application of long non-coding RNA LINC00842 as a biomarker in the preparation of prognostic detection preparations for lung adenocarcinoma
  • Application of long non-coding RNA LINC00842 as a biomarker in the preparation of prognostic detection preparations for lung adenocarcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 Preparation of long-chain non-coding RNA LINC00842 for lung adenocarcinoma prognosis detection kit (50 reactions)

[0014] 1. RNA stabilization solution 50ml

[0015] 2. Isopropanol 100ml

[0016] 3. Chloroform 100ml

[0017] 4. Trizol 50ml

[0018] 5. Enzyme-free water 10ml

[0019] 6.1μM random reverse transcription primer 50ul

[0020] 7. 5× reverse transcription buffer 200ml

[0021] 8. 10mM base triphosphate deoxyribonucleotides 100ul

[0022] 9. 40U / μl RNase inhibitor 500ul

[0023] 10. 200U / μl MMLV reverse transcriptase 50ul

[0024] 11. Premix Ex Taq 50ul

[0025] 12. 10μM long non-coding RNA LINC00842 real-time fluorescence quantitative PCR specific primers:

[0026] Forward primer 5'-CTGCCCAGTTAAGGCACAACTCTC-3' (SEQ ID NO.2),

[0027] Reverse primer 5'-AGCGCTGCGGAGAGGCAGTCT-3' (SEQ ID NO.3),

[0028] 13. 10μM internal reference GAPDH specific primers for real-time fluorescence quantitative PCR:

[0029] The forward primer is 5'-GTGGATATTG...

Embodiment 2

[0031] Example 2 Detection of long non-coding RNA LINC00842 in lung adenocarcinoma tissue

[0032] 1. Preservation of lung adenocarcinoma tissue: collect the lung adenocarcinoma tissue to be tested and store it in a cryopreservation tube filled with RNA stabilization solution, and put it in a -80°C refrigerator for later use.

[0033] 2. Extraction of RNA in tissues: Take an appropriate amount of specimen, add liquid nitrogen to the mortar after baking at 180°C for 6-8 hours, grind the specimen, grind to powder, add 1ml Trizol mortar specimen to the mortar, and grind into After the liquid is in the centrifuge tube, add 200μl / ml chloroform to the centrifuge tube, shake it by hand for 15-30s, place it on ice for 5min, and centrifuge at 12000g at 4°C for 15min; carefully take the upper aqueous phase into a new centrifuge tube, add Mix with 0.5ml of pre-cooled isopropanol, put it in a refrigerator at -20°C for 20min, centrifuge at 12000g at 4°C for 10min; discard the supernatant, ...

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Abstract

The invention discloses the application of long-chain non-coding RNA LINC00842 as a biomarker in the preparation of lung adenocarcinoma prognosis detection preparations. It can detect the expression level of long non-coding RNA LINC00842 in patients with lung adenocarcinoma, which provides a strong molecular biological basis for prognosis prediction of patients with lung adenocarcinoma, and has far-reaching clinical significance and promotion.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and specifically relates to the application of long-chain non-coding RNA LINC00842 as a biomarker in the preparation of lung adenocarcinoma prognosis detection reagents. Background technique [0002] In recent years, the incidence of lung cancer has been increasing. According to statistics from the World Cancer Research Foundation, newly diagnosed lung cancer cases worldwide account for 12.5% ​​of the total number of cancers every year. Globally, lung cancer kills 1 million lives every year. In China, lung cancer has ranked first in the death rate of various cancers for several consecutive years. Lung cancer is mainly divided into small cell lung cancer and non-small cell lung cancer according to different histological types, among which lung adenocarcinoma is the most common type of non-small cell lung cancer. Although the advancement of diagnosis and treatment technology, especially the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/166C12Q2600/178
Inventor 陶永光毛超王敏刘双
Owner XIANGYA HOSPITAL CENT SOUTH UNIV