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Primary lung adenocarcinoma molecular typing and survival risk gene group, diagnostic product and application

A technology for molecular typing and lung adenocarcinoma, which is applied in biochemical equipment and methods, analytical materials, microbial measurement/testing, etc., can solve the problems of great differences in treatment efficiency

Inactive Publication Date: 2021-03-05
SHANGHAI PRECISION DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, for the same treatment plan, the effective rate of different patients varies greatly

Method used

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  • Primary lung adenocarcinoma molecular typing and survival risk gene group, diagnostic product and application
  • Primary lung adenocarcinoma molecular typing and survival risk gene group, diagnostic product and application
  • Primary lung adenocarcinoma molecular typing and survival risk gene group, diagnostic product and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0123] Example 1: Evaluation of subtypes of lung adenocarcinoma and screening of survival risk-related gene groups

[0124] Methods: 504 cases in the TCGA database with complete According to the clinical information of lung adenocarcinoma gene expression, the proliferation-related genes, immune-related genes, and intercellular matrix-related genes that are closely related to the survival risk of lung adenocarcinoma are screened out, and the type and survival risk are calculated and optimized in each group of genes Genes with high contribution rate.

[0125] Results: A total of 180 genes and 6 housekeeping genes related to lung adenocarcinoma subtype and survival risk were screened, that is, 186 gene test combinations. See Table 1 for the list of genes.

[0126] The validity and stability of the 186 genes screened were verified in the Affymatrics gene chip expression profile data of 1346 cases of lung adenocarcinoma.

Embodiment 2

[0127] Example 2: Molecular typing and survival risk assessment of lung adenocarcinoma by next-generation sequencing

[0128]Experimental method: A 76-gene test combination was used, of which 70 lung adenocarcinoma subtypes and survival risk-related gene groups (proliferation-related genes: PLK1, PRC1, CCNB1, DLGAP5, KPNA2, CCNA2, RRM2, FOXM1, MKI67, KIF14, HJURP, TPX2, NEK2, CDK1, CDKN3, ASPM, CEP55, BIRC5, MELK, CDC20, TYMS, AURKA and TOP2A; immune-related genes: P2RY13, CCR2, PTPRC, IRF8, CLEC10A, TLR7, CCR4, IL7R, SPN, SASH3, CSF2RB, CD37, IKZF1, CD48, IL10RA, EVI2B, IGSF6, CD52, DOCK2, CD84, FOLR2, NCKAP1L, TRAC, MNDA, MRC1, PLEK, SPIB, CD53, CD4, and LYZ; cytoplasmic related genes: SPOCK1, COL1A1 , POSTN, ADAM12, COL6A2, COL5A1, COL11A1, COL5A2, COL1A2, MXRA5, THBS2, INHBA, VCAN, ADAMTS12, GREM1, COL3A1 and SULF1) for molecular typing and survival risk assessment, 6 internal reference genes (including GAPDH, GUSB , MRPL19, PSMC4, SF3A1 and TFRC) were used as internal st...

Embodiment 3

[0155] Example 3: Molecular classification and survival risk assessment of lung adenocarcinoma by quantitative PCR

[0156] Experimental method: A 25-gene test combination was used, of which 24 lung adenocarcinoma subtypes and survival risk-related gene groups (proliferation-related genes: PLK1, PRC1, CCNB1, MKI67, TPX2, MELK, CDC20, TYMS, and TOP2A; immune-related Genes: P2RY13, CCR2, PTPRC, IRF8, CLEC10A, TLR7, CCR4, IL7R and CD4; intercellular matrix-related genes: SPOCK1, COL1A1, POSTN, ADAM12, COL6A2 and COL5A1) for molecular typing and survival risk assessment, 1 An internal reference gene (ACTB) was used as an internal standard. All 24 genes in Table 3 except the internal reference genes were used to calculate the survival risk index.

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Abstract

The invention belongs to the technical field of biology, discloses a gene group capable of evaluating primary lung adenocarcinoma molecular typing and survival risk, and discloses application of reagents for detecting gene expression levels of the gene group in preparing in-vitro diagnostic products for evaluating primary lung adenocarcinoma molecular typing and survival risk. The in-vitro diagnostic products comprise a Next Generation Sequencing (NGS) detection kit, a fluorescent quantitative PCR detection kit, a gene chip and a protein array. The invention further discloses a method for evaluating primary lung adenocarcinoma molecular typing and survival risk by utilizing the detection kits. The method disclosed by the invention has high accuracy on evaluation of primary lung adenocarcinoma subtype typing and survival risk.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to subtyping of lung adenocarcinoma and assessing survival risk gene groups, in vitro diagnostic products and applications thereof. Background technique [0002] Lung cancer is one of the most common malignant tumors worldwide. The morbidity and mortality of lung cancer rank first among malignant tumors in my country. According to the 2015 Chinese cancer statistics published by the Cancer Hospital of the Chinese Academy of Medical Sciences, the National Cancer Center, and the National Cancer Registry Center in "CA: ACancer Journal for Clinicians" (impact factor 144.8), the number of new lung cancer cases in my country in 2015 was 733,000. The number of deaths was 610,000. The incidence and death rates in the United States during the same period were 221,000 and 158,000, respectively (https: / / www.cancer.org / research / cancer-facts-statistics). The incidence rate between the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158C40B40/06G01N33/574C12Q2600/16C12Q2600/112C12N15/111C12Q1/6869
Inventor 周彤周伟庆胡志元马琳琳
Owner SHANGHAI PRECISION DIAGNOSTICS CO LTD
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