A biological trace rapid visualization agent
A technique for developing agents and traces, applied in chemical/physical processes, chemical/physical/physical-chemical processes, chemical/physical/physical-chemical fixed reactors, etc., which can solve problems such as low clarity and resolution, DNA inspection damage, It takes a long time for developing and other problems, and achieves the effect of simple and convenient operation, fast display time and clear developing effect
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Embodiment 1
[0049] A biological trace rapid visualization agent is composed of the following raw materials: fluorescent substance 1kg, ninhydrin 4kg, lanthanum oxide 0.2kg, silver nitrate 3kg, polyether F68 0.1kg, ethanol 90kg; the pH of the biological trace rapid visualization agent is 4.5; the fluorescent substance is eosin Y.
[0050] The above-mentioned biological trace rapid visualization agent is prepared according to the following steps:
[0051] ① Add 1 kg of fluorescent substance to 45 kg of ethanol and stir until it is completely dissolved, then add 4 kg of ninhydrin to it until it is completely dissolved to obtain the first solution; the fluorescent substance is Eosin Y;
[0052] ② Add 3kg of silver nitrate and 0.2kg of lanthanum oxide into 45kg of ethanol, heat and stir until completely dissolved, and control the heating temperature within 65°C to obtain the second solution;
[0053] ③Mix the first solution obtained in step ① with the second solution obtained in step ② to obt...
Embodiment 2
[0055] A biological trace rapid visualization agent, consisting of the following raw materials: fluorescent substance 2kg, ninhydrin 8kg, lanthanum oxide 1kg, silver nitrate 10kg, polyether F68 0.4kg, ethanol 110kg; the pH of the biological trace rapid visualization agent is 6.5 ; The fluorescent substance is fluorescein.
[0056] The above-mentioned biological trace rapid visualization agent is prepared according to the following steps:
[0057] ①Add 2kg of fluorescent substance into 55kg of ethanol and stir until completely dissolved, then add 8kg of ninhydrin until completely dissolved to obtain the first solution; the fluorescent substance is fluorescein;
[0058] ② Add 10kg of silver nitrate and 1kg of lanthanum oxide into 55kg of ethanol, heat and stir until completely dissolved, and control the heating temperature within 65°C to obtain the second solution;
[0059] ③Mix the first solution obtained in step ① with the second solution obtained in step ② to obtain a mixed ...
Embodiment 3
[0061] A biological trace rapid visualization agent, consisting of the following raw materials: fluorescent substance 1.5kg, ninhydrin 7kg, lanthanum oxide 0.6kg, silver nitrate 8kg, polyether F68 0.2kg, ethanol 105kg; the pH of the biological trace rapid visualization agent is 5.5; the fluorescent substance is calcein.
[0062] The above-mentioned biological trace rapid visualization agent is prepared according to the following steps:
[0063] ①Add 1.5kg of fluorescent substance to 52.5kg of ethanol and stir until completely dissolved, then add 7kg of ninhydrin to it until completely dissolved to obtain the first solution; the fluorescent substance is calcein;
[0064] ② Add 8kg of silver nitrate and 0.6kg of lanthanum oxide into 52.5kg of ethanol, heat and stir until completely dissolved, and control the heating temperature within 65°C to obtain the second solution;
[0065]③Mix the first solution obtained in step ① with the second solution obtained in step ② to obtain a mi...
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